{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T12:25:12Z","timestamp":1771590312980,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,8,7]],"date-time":"2005-08-07T00:00:00Z","timestamp":1123372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to investigate the molecular changes that underlie morphological changes in the epididymis following neonatal exposure to potent synthetic estrogen, namely diethylstilbestrol (DES). Newborn male mice were subcutaneously injected with DES or endogenous estrogen, namely 17 \u03b2\u2010estradiol (E2) (5 \u00b5g\/mouse\/day), for the first 5 days. At the age of 2, 4, and 8 weeks, epididymides of the mice were dissected. Characteristic morphological abnormality, such as relative stromal overgrowth, was observed at the age of 2 weeks in the epididymis of DES\u2010treated mice, but not in E2\u2010treated mice. Microarray and real\u2010time RT\u2010PCR analyses revealed that the expression levels of procollagen type I alpha 1 (col1a1) and col1a2 genes were markedly upregulated at the age of 2 weeks in the epididymis of DES\u2010treated mice in comparison with the control. Western blot analysis revealed that type I collagen protein expression level in epididymis of DES\u2010treated mice was elevated at the age of 2 weeks. In situ hybridization analysis revealed that the signals of col1a1 mRNA were detected similarly throughout the stromal tissue of epididymis at the age of 2 weeks in control and DES\u2010 and E2\u2010treated mice. The gene expression level of epididymal type III collagen (col3a1), which is found in many stromal connective tissues as well as type I collagen, did not change at the age of 2 weeks in all groups. These results suggest that the increased type I collagen expression is associated with the relative stromal overgrowth in the epididymis of DES\u2010treated mice. Mol. Reprod. Dev. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20347","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T06:16:32Z","timestamp":1123481792000},"page":"291-298","source":"Crossref","is-referenced-by-count":18,"title":["Association of increased type I collagen expression and relative stromal overgrowth in mouse epididymis neonatally exposed to 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