{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:56:56Z","timestamp":1767855416939,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2007,3,15]],"date-time":"2007-03-15T00:00:00Z","timestamp":1173916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study investigated the effect of treatment with 6\u2010dimethylaminopurine (6\u2010DMAP) following fusion on in vitro development of porcine nuclear transfer (NT) embryos. Frozen thawed ear skin cells were transferred into the perivitelline space of enucleated oocytes. Reconstructed oocytes were fused and activated with electric pulse in 0.3 M mannitol supplemented with either 0.1 or 1.0 mM CaCl<jats:sub>2<\/jats:sub>. In each calcium concentration, activated oocytes were divided into three groups. Two groups of them were exposed to either ionomycin (I\u2009+\u20096\u2010DMAP or 6\u2010DMAP alone. In experiment 2, fused NT embryos in 0.3 M mannitol containing 1.0 mM CaCl<jats:sub>2<\/jats:sub> were exposed to 6\u2010DMAP either immediately or 20 min after fusion\/activation. For 0.1 mM CaCl<jats:sub>2<\/jats:sub>, oocytes activated with either I\u2009+\u20096\u2010DMAP or 6\u2010DMAP alone showed a higher (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) developmental rate to the blastocyst stage than those activated with an electric pulse alone (26.7 and 22.5 vs. 12.5%). For 1.0 mM CaCl<jats:sub>2<\/jats:sub>, oocytes activated with either I\u2009+\u20096\u2010DMAP or 6\u2010DMAP alone showed significantly higher (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) developmental rate to the blastocyst stage (35.6 and 28.3 vs. 19.8%). Developmental rate to the blastocyst stage was (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) increased in NT embryos activated with 6\u2010DMAP 20 min after fusion. 6\u2010DMAP made a higher and wider Ca<jats:sup>2+<\/jats:sup> transient compared to that induced by electric pulses (Fig. 3). The fluctuation lasted during the time that oocytes were cultured in 6\u2010DMAP. Regardless of Ca<jats:sup>2+<\/jats:sup> concentration in fusion medium, activation with 6\u2010DMAP following electric pulses supported more development of porcine NT embryos. Activation of NT embryos with 6\u2010DMAP after fusion in the presence of 1.0 mM CaCl<jats:sub>2<\/jats:sub> could support better developmental rate to the blastocyst stage. Mol. Reprod. Dev. 74: 1158\u20131164, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20492","type":"journal-article","created":{"date-parts":[[2007,3,15]],"date-time":"2007-03-15T17:37:51Z","timestamp":1173980271000},"page":"1158-1164","source":"Crossref","is-referenced-by-count":14,"title":["Development and calcium level changes in pre\u2010implantation porcine nuclear transfer embryos activated with 6\u2010DMAP after fusion"],"prefix":"10.1002","volume":"74","author":[{"given":"Gi\u2010Sun","family":"Im","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa","family":"Samuel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangxue","family":"Lai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhong","family":"Hao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randall S.","family":"Prather","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/80242"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1081\/ABIO-100108347"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD00051"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10110"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5367.1256"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080340214"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/15204559950020102"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD00033"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10126"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2003.06.006"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1242\/dev.111.3.813","article-title":"Tyrosine phosphorylation of p34cdc2 and p42 during meiotic maturation of Xenopus oocyte. 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