{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:24:15Z","timestamp":1778657055136,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2006,5,16]],"date-time":"2006-05-16T00:00:00Z","timestamp":1147737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study was undertaken to determine the effects of gonadotrophin on cytoskeletal dynamics and embryo development and its role in improving the retrieval of developmentally competent oocytes. Female golden hamsters were injected with human chorionic gonadotrophin (hCG; 5\u2010, 7.5\u2010 or 15\u2010IU) on the day 4 of estrus, pregnant mare serum gonadotrophin (PMSG; 5\u2010, 7.5\u2010 or 15\u2010IU) on the day 1 of estrus, or 15\u2010IU hCG at 56 hr post\u201015\u2010IU PMSG injection in any cycle except estrus. Increasing the hCG dose decreased not only retrieval rate of 2\u2010cell embryo but development to blastocyst after subsequent in vitro culture. Whereas, although increasing the PMSG dose induced increasing the number of 2\u2010cell embryo and blastocyst, 15\u2010IU PMSG injection caused retardation of development to blastocyst. No 2\u2010cell embryos were retrieved by injecting both PMSG and hCG. The injections of 15\u2010IU hCG and 7.5\u2010 or 15\u2010IU PMSG inhibited the proliferation of trophectodermal and inner cell mass cells, respectively. Gonadotrophin injection didn't influence microtubular spindle formation, but 5\u2010 or 15\u2010IU hCG, 15\u2010IU PMSG, or PMSG and hCG injections induced aberrant cortical granule (CG) and microfilament distribution. After 15\u2010IU hCG or PMSG and hCG injections, fewer oocytes had enriched cortical actin domains, and the expression of \u03b1\u2010, \u03b2\u2010 and \u03b3\u2010actin genes was greatly increased. In conclusion, a high dose of gonadotrophins alters the microfilament and CG distribution, which in turn reduces the developmental competence of oocytes. Injecting a reduced dose of PMSG to initiate ovarian hyperstimulation without triggering ovulation contributes to the efficient retrieval of developmentally competent oocytes. Mol. Reprod. Dev. 1022\u20131033, 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20500","type":"journal-article","created":{"date-parts":[[2006,5,16]],"date-time":"2006-05-16T22:56:11Z","timestamp":1147820171000},"page":"1022-1033","source":"Crossref","is-referenced-by-count":21,"title":["Influence of ovarian hyperstimulation and ovulation induction on the cytoskeletal dynamics and developmental competence of oocytes"],"prefix":"10.1002","volume":"73","author":[{"given":"Seung Tae","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ho Jae","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seo Jin","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun Ju","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae 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