{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T06:04:01Z","timestamp":1743746641197},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,8,29]],"date-time":"2006-08-29T00:00:00Z","timestamp":1156809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ability of synthetic arginine\u2010glycine\u2010aspartic acid (RGD)\u2010containing peptides to induce intracellular calcium transients similar to those observed at fertilization by spermatozoa in the bovine has been reported (Campbell et al., 2000: Biol Reprod 62:1702\u20131709; Sessions et al., 2006. Mol Reprod Dev). These results also indicated the ability of synthetic RGD\u2010containing peptides to induce activation and subsequent parthenogenetic development to the blastocyst stage, although, at numbers lower than observed with control in vitro fertilization (IVF). Evidence has been provided indicating the important effect of surrounding regions on the biological activity of the RGD sequence (Zhu and Evans, 2002; Sessions et al., 2006). The current experiments were designed to use natural RGD\u2010containing sequences (disintegrins) to understand their effects. A total of three RGD\u2010containing snake venom peptides (Kistrin (K), Elegantin (Ele), and Echistatin (Ech)) and one nonRGD\u2010containing venom (Erabutoxin B (EB; control) were used at three concentrations (0.1, 1, and 10 \u00b5g \/ml) to induce parthenogenetic development to the blastocyst stage and in conjunction (1.0, 5.0, and 10 \u00b5g\/ml) with spermatozoa to evaluate competitive inhibition of fertilization and subsequent development. A (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) higher number of bovine oocytes developed to the blastocyst stage after incubation with K, Ele and Ech at 1.0 \u00b5g\/ml, and was not different (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.01) from IVF control. Fertilization was significantly reduced (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) at all concentrations of K, Ele and Ech as compared to IVF control. No reduction (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05) was observed in EB (nonRGD) treated oocytes. These results support the involvement of a disintegrin\u2013integrin interaction at fertilization in the bovine resulting in activation and subsequent development. Mol. Reprod. Dev. 74: 88\u201396, 2007. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20522","type":"journal-article","created":{"date-parts":[[2006,8,29]],"date-time":"2006-08-29T22:55:50Z","timestamp":1156892150000},"page":"88-96","source":"Crossref","is-referenced-by-count":9,"title":["Effects of arginine\u2010glycine\u2010aspartic acid (RGD) containing snake venom peptides on parthenogenetic development and in vitro fertilization of bovine oocytes"],"prefix":"10.1002","volume":"74","author":[{"given":"K.L.","family":"White","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Passipieri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.D.","family":"Bunch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.D.","family":"Campbell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Pate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1862345"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(05)80014-5"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.21.10051"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120230202"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/356248a0"},{"key":"e_1_2_1_7_1","first-page":"213","article-title":"Evidence for the role of fibronectin in amphibian gastrulation","volume":"89","author":"Boucaut JC","year":"1985","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod43.6.1019"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)53775-3"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)57744-9"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)01203-9"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.6.1702"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"3267","DOI":"10.1242\/jcs.108.10.3267","article-title":"Mouse sperm\u2010egg plasma membrane interactions: Analysis of roles of egg integrins and the mouse sperm homologue of PH\u201030 (fertilin) beta","volume":"108","author":"Evans JP","year":"1995","journal-title":"J Cell Sci"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8611"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1226"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod53.4.766"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0810077"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1992.tb00342.x"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080310309"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080360212"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"19827","DOI":"10.1016\/S0021-9258(19)77710-2","article-title":"Echistatin: A potent platelet aggregation inhibitor from the venom of the viper, Echis carinatus","volume":"25","author":"Gan ZR","year":"1988","journal-title":"J Biol Chem"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.117.2.449"},{"key":"e_1_2_1_23_1","first-page":"105","article-title":"Beta1 integrin ligands of spermatozoa: A flow cytophometric analysis","volume":"16","author":"Gladner HJ","year":"1983","journal-title":"Inter J Andro"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-195-43129B"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(94)90091-4"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.3109\/15419069309095680"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050181"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90115-S"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:19960207"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.0185"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.20.13094"},{"key":"e_1_2_1_32_1","doi-asserted-by":"crossref","first-page":"4471","DOI":"10.1016\/S0021-9258(20)64346-0","article-title":"Calcium as a potential physiological regulator of integrin\u2010mediated cell adhesion","volume":"266","author":"Kirchhofer D","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_1_33_1","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1093\/oxfordjournals.humrep.a136023","article-title":"A positive correlation between expression of beta 1\u2010integrin cell adhesion molecules and fertilizing ability of human spermatozoa in vitro","volume":"10","author":"Klentzeris LD","year":"1995","journal-title":"Hum Reprod"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(92)90265-I"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(96)76591-8"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8392749"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1530\/reprod\/120.1.65"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod59.3.537"},{"key":"e_1_2_1_39_1","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1042\/bj3040929","article-title":"Preferential antagonism of the interactions of the integrin \u03b1 IIb\u03b23 with immobilized glycoprotein ligands by snake\u2010venom RGD (Arg\u2010Gly\u2010Asp) proteins","volume":"304","author":"Lu X","year":"1994","journal-title":"Biochemistry"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-219-44322"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.18.9.1363"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.41.77"},{"key":"e_1_2_1_43_1","first-page":"1152","volume-title":"Lehninger principles of biochemistry","author":"Nelson DL","year":"2000"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1179"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(92)90040-C"},{"key":"e_1_2_1_46_1","first-page":"237","article-title":"Specific integrin subunits present on the vitelline membrane of bovine oocytes. 37rd Annual Meeting of the Society for the Study of Reproduction, Vancouver, BC","volume":"71","author":"Pate BJ","year":"2004","journal-title":"Biol Reprod"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod60.3.747"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.1.137"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2420006"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1304"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(95)00380-Q"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114957"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2821619"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00244a003"},{"key":"e_1_2_1_55_1","doi-asserted-by":"crossref","first-page":"9359","DOI":"10.1016\/S0021-9258(18)92826-7","article-title":"Barbourin a GPIIb\u2010IIIa\u2010specific integrin antagonist from the venom of Sistrurus m. barbouri","volume":"266","author":"Scarborough RM","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20462"},{"key":"e_1_2_1_57_1","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1242\/dev.115.4.947","article-title":"A comparison of intracellular changes in porcine oocytes after fertilization and electroactivation","volume":"115","author":"Sun FZ","year":"1992","journal-title":"Development"},{"key":"e_1_2_1_58_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1010713"},{"key":"e_1_2_1_59_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119.4.1175"},{"key":"e_1_2_1_60_1","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1242\/dev.117.4.1369","article-title":"Expression of beta 1 integrin complexes on the surface of unfertilized mouse oocyte","volume":"117","author":"Tarone G","year":"1993","journal-title":"Development"},{"key":"e_1_2_1_61_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.121.2.461"},{"key":"e_1_2_1_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)98761-2"},{"key":"e_1_2_1_63_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod19.5.949"},{"key":"e_1_2_1_64_1","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1242\/dev.121.8.2645","article-title":"The existence of inositol 1,4,5\u2010trisphosphate and ryanodine receptors in mature bovine oocytes","volume":"121","author":"Yue C","year":"1996","journal-title":"Development"},{"key":"e_1_2_1_65_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.4.1193"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.20522","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.20522","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T11:30:55Z","timestamp":1697110255000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.20522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8,29]]},"references-count":64,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1002\/mrd.20522"],"URL":"https:\/\/doi.org\/10.1002\/mrd.20522","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,8,29]]}}}