{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:39:57Z","timestamp":1767832797684,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,11,8]],"date-time":"2006-11-08T00:00:00Z","timestamp":1162944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cloning by somatic nuclear transfer is an inefficient process in which many of the cloned animals die shortly after birth and display organ abnormalities. In an effort to determine the possible roles IGFs played in neonatal death and organ abnormalities, we have examined expression patterns of eight genes in insulin\u2010like growth factor (IGF) systems (<jats:italic>IGF1, IGF2, IGF1R, IGF2R, IGFBP\u20101, IGFBP\u20102, IGFBP\u20103,<\/jats:italic> and <jats:italic>IGFBP\u20104<\/jats:italic>) in six organs (heart, liver, spleen, lung, kidney, and brain) of both neonatal death cloned bovines (n\u2009=\u20099) and normal control calves (n\u2009=\u20093) produced by artificial insemination (AI) using real\u2010time quantitative RT\u2010PCR. The effect of the age of the fibroblast donor cell on the gene expression profiles was also investigated. Aberrant expressions of six genes (<jats:italic>IGF2, IGF1R, IGF2R, IGFBP\u20102, IGFBP\u20103,<\/jats:italic> and <jats:italic>IGFBP\u20104<\/jats:italic>) were found in some studied tissues, but the expression of two genes (<jats:italic>IGF1<\/jats:italic> and <jats:italic>IGFBP\u20101<\/jats:italic>) had similar levels with the normal controls. For the studied genes, kidney was the organ that was most affected (five genes) by gene downregulation, whereas spleen was the organ that was not affected. The two upregulation genes were in brain, but both of downregulation and upregulation were found in the heart, liver, and lung. The expression of three genes (<jats:italic>IGF2R<\/jats:italic>, <jats:italic>IGFBP\u20104,<\/jats:italic> and <jats:italic>IGF2<\/jats:italic>) in some tissues showed significant differences between AF cell\u2010derived and FF cell\u2010derived clones. Our results suggest that aberrations in gene expression within IGF systems were found in most cloned bovine tissues of neonatal death. Because IGF systems play an important role in embryo development and organogenesis, the aberrant transcription patterns detected in these clones may contribute to the defects of organs reported in neonatal death of clones. Mol. Reprod. Dev. 74: 397\u2013402, 2007. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20534","type":"journal-article","created":{"date-parts":[[2006,11,8]],"date-time":"2006-11-08T22:17:25Z","timestamp":1163024245000},"page":"397-402","source":"Crossref","is-referenced-by-count":22,"title":["Expression of insulin\u2010like growth factors systems in cloned cattle dead within hours after 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