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Within its complex structure, the mammalian sperm flagellum contains F\u2010actin and thus, we decided to test in the guinea pig sperm flagellum the role of F\u2010actin in motility. During maturation, capacitation, and the acrosome reaction, a gradual decrease of the relative concentration of F\u2010actin was observed. Motility increased as spermatozoa became able to fertilize. Gelsolin, phalloidin, and KI inhibited sperm motility. Gelsolin canceled sperm motility within 20 min of treatment while 0.6 M KI had immediate effects. Phalloidin diminished hyperactive sperm motility slightly. All three compounds significantly increased the relative concentration of F\u2010actin. Latrunculins are conventional drugs that destabilize the F\u2010actin cytoskeleton. Latrunculin A (LAT A) did not affect sperm motility; but significantly increased F\u2010actin relative concentration. The results suggested that in guinea pig spermatozoa, randomly severing F\u2010actin filaments inhibits flagellar motility; while end filament alteration does not. Thus, specific filament regions seem to be important for sperm motility. Mol. Reprod. Dev. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20578","type":"journal-article","created":{"date-parts":[[2006,9,22]],"date-time":"2006-09-22T17:56:48Z","timestamp":1158947808000},"page":"312-320","source":"Crossref","is-referenced-by-count":33,"title":["F\u2010actin involvement in guinea pig sperm motility"],"prefix":"10.1002","volume":"74","author":[{"given":"Yenia","family":"Azamar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvador","family":"Uribe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adela","family":"M\u00fajica","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,9,22]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"32916","DOI":"10.1016\/S0021-9258(20)30078-8","article-title":"Gelsolin displaces phalloidin from actin filaments. 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