{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:09:34Z","timestamp":1760645374106},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T00:00:00Z","timestamp":1173139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Expression of <jats:italic>T\u2010complex testis expressed 5<\/jats:italic> (<jats:italic>Tctex5<\/jats:italic>), an orthologue of protein phosphatase\u20101 inhibitor\u20103 (<jats:italic>PPP1R11<\/jats:italic>), was enhanced in mouse testis and was also expressed in epididymis and spermatozoa. There were three transcripts of <jats:italic>Tctex5<\/jats:italic> including one brain specific and two common transcripts dominant in mouse testis. Tctex5 protein isoforms (75, 52, 32, 25, and 14.3 kDa) were identified. Isoforms of 75 and 52 kDa were spermatogenic\u2010specific and were found in protein fraction containing nuclei, mitochondria, and flagellum accessory, and also in protein fraction containing mainly membranes. Tctex5 was localized in nuclei of pachytene spermatocytes, round spermatocytes, cytoplasm of Sertoli cells in testis; cilia, secretion bodies and nuclei of epithelial cells and interstitium smooth muscle cells in epididymis; and head and principal piece of tail in epididymal spermatozoa. The results suggested that <jats:italic>Tctex5<\/jats:italic> might be a specific protein phosphatase\u20101 inhibitor in sperm; various <jats:italic>Tctex5<\/jats:italic> transcripts and isoforms and cellular locations imply its different roles in spermatogenesis. Nuclei\u2010type isoforms (75 and 52 kDa) might take part in nucleus remodeling during spermatogenesis whilst membrane\u2010type isoform (52 kDa) might be responsible for dephosphorylation of proteins during capacitation. The other isoforms might play general roles for all kinds of cell types. Mol. Reprod. Dev. 74: 1132\u20131140, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20631","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T17:41:25Z","timestamp":1173202885000},"page":"1132-1140","source":"Crossref","is-referenced-by-count":13,"title":["Expression of a novel <i>T\u2010complex testis expressed 5<\/i> (<i>Tctex5<\/i>) in mouse testis, epididymis, and spermatozoa"],"prefix":"10.1002","volume":"74","author":[{"given":"Y.B.","family":"Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.L.","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.K.","family":"Cheung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.M.","family":"Lam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.C.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.J.","family":"Haines","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(00)00069-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(01)01836-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409239209082564"},{"key":"e_1_2_1_5_1","first-page":"197","article-title":"Protein phosphatases","volume":"49","author":"Brautigan DL","year":"1994","journal-title":"Recent Prog Horm Res"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M500322-MCP200"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00013.2003"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206838200"},{"key":"e_1_2_1_9_1","unstructured":"ChengL MyersK HanY PilderSH VijayaraghavanS.2005.T complex protein tectex5 is a candidate for the protein phosphatase 1 gamma 2 inhibitor in spermatozoa. 38th Annual Meeting of Society for the Studies of Reproduction. 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