{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:02:01Z","timestamp":1790524921634,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,10,30]],"date-time":"2006-10-30T00:00:00Z","timestamp":1162166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Survivin, an inhibitor of apoptotic protein containing a single baculoviral inhibit apoptotic protein repeat domain, is a bifunctional protein that suppresses apoptosis and regulates cell division. Thus, we used double stranded RNA (dsRNA) interference to manipulate survivin expression in bovine embryos and analyze its role in blocking apoptosis and facilitating development of pre\u2010implantation embryos. In vitro fertilized embryos (1\u2010cell) were injected with survivin dsRNA, and expression of survivin mRNA was evaluated by real\u2010time quantitative RT\u2010PCR. To analyze survivin protein expression, we performed immunocytochemistry using a rabbit anti\u2010bovine suvivin antibody. Expression levels of survivin mRNA and protein were decreased in the dsRNA group compared to the sham group. Rates of in vitro blastocyst development were lower in the survivin dsRNA\u2010injected group than in the sham\u2010injected group. Also, the total cell number seen in blastocysts was decreased in the dsRNA group. TUNEL assays of DNA fragmentation indicated an increased apoptotic index in the dsRNA group compared to the sham group. These results indicate that survivin is important for optimal development of bovine blastocysts and confirm that survivin expression suppresses apoptosis of pre\u2010implantation embryos. Mol. Reprod. Dev. 74: 582\u2013590, 2007. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20641","type":"journal-article","created":{"date-parts":[[2006,10,30]],"date-time":"2006-10-30T20:18:47Z","timestamp":1162239527000},"page":"582-590","source":"Crossref","is-referenced-by-count":16,"title":["Survivin acts as anti\u2010apoptotic factor during the development of bovine pre\u2010implantation embryos"],"prefix":"10.1002","volume":"74","author":[{"given":"Sae Young","family":"Park","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Young","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kilsoo","family":"JeOn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang\u2010Shun","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won Don","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam\u2010Hyung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se Pill","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Ho","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,10,30]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"43","article-title":"Developmentally regulated expression of the novel cancer antiapoptotic gene survivin in human and mouse differentiation","volume":"152","author":"Adida C","year":"1998","journal-title":"Am J Pathol"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0897-917"},{"key":"e_1_2_1_4_1","volume-title":"SAS Users Guide. 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