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NSSR1 was mainly expressed in germ cells, but barely detected in Sertoli cells. Testicular NSSR1 was mostly phosphorylated and cytosolic in germ cells. In comparison, pituitary NSSR1 was mostly dephosphorylated and nuclear. In the cryptorchid testes, the dephosphorylated NSSR1 was significantly increased. RT\u2010PCR analysis demonstrated that the alternative splicing of CREB and CREM genes was altered in the cryptorchid testes. In addition, CREB transcripts were associated with NSSR1 either in testes tissues or cultured GC\u20101 cells. Moreover, the studies with NSSR1 over\u2010expression or silence demonstrated that NSSR1 promoted the exon 5 inclusion of CREB, indicating that NSSR1 is a new factor that regulates the alternative exon 5 inclusion of CREB transcripts. The findings for the first time provide the evidence indicating the potential importance of NSSR1 in testes development, spermatogenesis and cryptorchidism. Mol. Reprod. Dev. 74: 1363\u20131372, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20719","type":"journal-article","created":{"date-parts":[[2007,4,11]],"date-time":"2007-04-11T22:17:58Z","timestamp":1176329878000},"page":"1363-1372","source":"Crossref","is-referenced-by-count":14,"title":["NSSR1 is regulated in testes development and cryptorchidism and promotes the exon 5\u2010included splicing of CREB 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