{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:16:24Z","timestamp":1772756184542,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,7,11]],"date-time":"2007-07-11T00:00:00Z","timestamp":1184112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CD52 is a human glycosylphosphatidylinositol (GPI)\u2010anchored antigen exclusively expressed in leukocytes and epididymal cells. It is also present in sperm, being inserted in their plasma membrane as they pass through the epididymis. In a previous paper we identified a new CD52 form without GPI anchor by fast performance liquid chromatography (FPLC) fractionation of semen components. The form has a lower negative charge than the GPI\u2010anchored form and occurs as the only CD52 form in prostasome\u2010free seminal plasma. It was also found associated with the ejaculated sperm, but in contrast to the GPI\u2010anchored one, it is lost during the capacitation process. In this paper we indicate that (1) the GPI\u2010anchored CD52 of the sperm surface serves as receptor for semenogelin I during clot formation, (2) liquefaction involves cleavage of the GPI anchor from certain CD52 molecules, releasing sperm from the clot and the soluble antigen bound to semenogelin fragments into the seminal plasma and (3) the clot is a sponge\u2010like structure housing sperm. Soluble CD52 was immunopurified from the soluble CD52\u2010containing FPLC fraction using CAMPATH\u20101G and was found to be complexed with a semenogelin\u2010derived peptide of the carboxyl terminal portion of semenogelin I, having the sequence SQTEKLVAGKQI and starting from amino acid 376. Immunoprecipitation and immunoblot analyses using CAMPATH\u20101G and anti\u2010semenogelin as immunoprecipitating antibodies and anti\u2010gp20 and anti\u2010semenogelin as immunoblot detectors of the corresponding antigens, confirmed that the soluble CD52 formed a complex with semenogelin. The semenogelin\u2010CD52 soluble form was found to be a direct consequence of the liquefaction process since only the GPI\u2010anchored CD52 was recovered in uniquefied semen after recovering sperm and seminal plasma by urea solubilization of the clot. Mol. Reprod. Dev. 75: 326\u2013335, 2008. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20738","type":"journal-article","created":{"date-parts":[[2007,7,11]],"date-time":"2007-07-11T16:18:21Z","timestamp":1184170701000},"page":"326-335","source":"Crossref","is-referenced-by-count":19,"title":["The GPI\u2010anchored CD52 antigen of the sperm surface interacts with semenogelin and participates in clot formation and liquefaction of human semen"],"prefix":"10.1002","volume":"75","author":[{"given":"Federica","family":"Flori","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo","family":"Ermini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni Battista","family":"La Sala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessia","family":"Nicoli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonietta","family":"Capone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riccardo","family":"Focarelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Floriana","family":"Rosati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cinzia Della","family":"Giovampaola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,11,26]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1982.tb07125.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150080203"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)69848-0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1065"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.13.11.1303"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.10.082"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2005.00463.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.1093\/oxfordjournals.humrep.a135787","article-title":"Changes in the sialylglycoconjugate distribution on the human sperm surface during in\u2010vitro capacitation: Partial purification of a 20 kDa sialylglycoprotein of capacitated spermatozoa","volume":"10","author":"Focarelli R","year":"1995","journal-title":"Hum Reprod"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/4.2.119"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0554"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/5.1.46"},{"key":"e_1_2_1_13_1","unstructured":"GerritsenVB.2005.Shackled sperm. 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