{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T05:14:30Z","timestamp":1791695670805,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The possibility that differences in hormonal regimes between the two oviducts in the cow around ovulation affects secretory activity of the oviduct epithelial cells and\/or sperm\u2013oviduct binding was studied. Oviducts were collected immediately after slaughter at 6 hr before to 5 hr after timed ovulation of 14 normally cyclic cows that had been inseminated (n\u2009=\u20096) or not (n\u2009=\u20098) and material obtained from the same cows was processed in three ways. First, in vivo, after artificial insemination of the cows, low numbers of sperm cells (approx. 15 per oviduct) were found within the entire oviducts as observed by scanning electron microscopy (SEM). Almost all sperm were located in the isthmus and then only on ciliated cells and showed without exception fully matured, intact morphology. Secretory activity of noninseminated oviduct epithelia was induced after ovulation which was most predominant in the pockets of the ipsi\u2010lateral ampulla compared to the contra\u2010lateral ampulla (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). Second, ex vivo, explants dissected from oviducts of the noniseminated cows were incubated with sperm. In all cases, the sperm bound to the explants in a similar pattern as observed in vivo and this binding was strictly fucose\u2010dependent. The main difference with in vivo experiments was the high numbers of sperm bound at any site of the oviduct (\u223c3,000 cells per mm<jats:sup>2<\/jats:sup>) indicating the high sperm binding capacity of the oviduct epithelia. Ovulation induced a striking drop in sperm binding capacity in the oviducts and was most pronounced in the isthmus (\u223c1,300 cells per mm<jats:sup>2<\/jats:sup>;<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) and to a lesser extent in the ampulla (\u223c2,000 cells per mm<jats:sup>2<\/jats:sup>,<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). Third, in vitro, pieces of tissue dissected from oviducts of the noninseminated cows were cultured to mono\u2010layers. Culturing epithelial cells resulted in loss of their normal morphological appearance. In all cases, the sperm binding capacity in monolayers was very low (&lt;50 cells per mm<jats:sup>2<\/jats:sup>) when compared to corresponding explants (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). Sperm binding to monolayers originating from the isthmus (&lt;25 cells per mm<jats:sup>2<\/jats:sup>) was lower than in those from the ampulla (40\u201350 cells per mm<jats:sup>2<\/jats:sup>;<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) and remained similar after ovulation. In all three approaches, no significant differences were found in sperm\u2013oviduct binding characteristics and sperm\u2010distribution in the ipsi\u2010 versus contra\u2010lateral oviducts. This indicates, that systemic endocrine changes around ovulation rather than specific oviduct changes at the ipsi\u2013lateral oviduct induce secretion in oviduct epithelial cells, and thus induce sperm release. Mol. Reprod. Dev. 75: 60\u201374, 2008. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20766","type":"journal-article","created":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T15:16:49Z","timestamp":1180711009000},"page":"60-74","source":"Crossref","is-referenced-by-count":73,"title":["Sperm binding properties and secretory activity of the bovine oviduct immediately before and after ovulation"],"prefix":"10.1002","volume":"75","author":[{"given":"Edita","family":"Sostaric","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steph J.","family":"Dieleman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris H.A.","family":"van de Lest","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Colenbrander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter L.A.M.","family":"Vos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuria","family":"Garcia\u2010Gil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart M.","family":"Gadella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,6]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"743","article-title":"The mammalian oviductal epithelium: Regional variations in cytological and functional aspects of the oviductal secretory cells","volume":"11","author":"Abe H","year":"1996","journal-title":"Histol Histopathol"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092350309"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00327983"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/12.9.1985"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod67.4.1125"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod52.1.156"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0690641"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0970031"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.2460\/ajvr.1993.54.09.1505","article-title":"Capacitation\u2010like membrane changes and prolonged viability in vitro of equine spermatozoa cultured with uterine tube epithelial cells","volume":"54","author":"Ellington JE","year":"1993","journal-title":"Am J Vet Res"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00190108"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod59.6.1530"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1020087"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1050057"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.6.1754"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(87)80173-X"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:19840508"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-2243(83)90264-2"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(91)90020-T"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(91)90150-C"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402600212"},{"key":"e_1_2_1_22_1","first-page":"137","article-title":"A formaldehyde\u2010glutaraldehyde fixative of high osmolality for use in electron microscopy","volume":"27","author":"Karnovsky MJ","year":"1965","journal-title":"J Cell Biol"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jmicro.a023434"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01291-8"},{"key":"e_1_2_1_25_1","first-page":"433","volume-title":"Histological and histochemical methods. 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