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The VEGF\u2010receptor system was localized in epithelial and stromal cells, blood vessels, and myometrium. Western blot analysis showed higher levels of VEGF protein during the periovulatory and periimplantation periods (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001, and <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05, respectively). Constant expression of VEGF mRNA during the cycle and significant upregulation on Days 22\u201325 of gestation (vs. Days 9\u201317; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) was observed. Stable levels of VEGFR\u20101 mRNA and protein were detected in the endometrium of cyclic animals. However, higher VEGFR\u20101 protein expression was found on Days 16\u201317 of the estrous cycle (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) and Days 13\u201315 of gestation (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Protein expression of VEGFR\u20102 was elevated on Days 2\u20134 of the estrous cycle (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001), but mRNA levels were constant during the cycle. In pregnancy, VEGFR\u20102 protein expression started to increase after Day 15 (vs. Days 9\u201312; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), but induction of VEGFR\u20102 mRNA expression occurred earlier on Days 13\u201315. It appears from the present study that the VEGF\u2010receptor system is regulated in a temporal and spatial manner during the estrous cycle and early pregnancy in pigs. The results suggest that VEGF\u2010A family members are probably involved in appropriate preparation of endometrium for implantation and in vascular events during implantation in pigs. Mol. Reprod. Dev. 75: 362\u2013372, 2008. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20815","type":"journal-article","created":{"date-parts":[[2007,9,14]],"date-time":"2007-09-14T16:17:42Z","timestamp":1189786662000},"page":"362-372","source":"Crossref","is-referenced-by-count":45,"title":["Expression of the vascular endothelial growth factor\u2010receptor system in the porcine endometrium throughout the estrous cycle and early pregnancy"],"prefix":"10.1002","volume":"75","author":[{"given":"Monika M.","family":"Kaczmarek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Waclawik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Blitek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna 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