{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T18:07:35Z","timestamp":1775239655566,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,11,16]],"date-time":"2007-11-16T00:00:00Z","timestamp":1195171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated cAMP content, gap junctional communications (GJCs) status, and LH\u2010receptor (LH\u2010R) expression in porcine cumulus\u2013oocyte complexes (COCs) during in vitro maturation treated with the phosphodiesterase (PDE) inhibitor 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX) or with FSH. COCs were cultured for 20 hr (1st culture) in M199 containing 10% FBS (basic medium, BM group) or BM supplemented with FSH (FSH group) or IBMX (IBMX group). Each COC was then transferred into BM containing both FSH and LH and cultured for an additional 24 hr (2nd culture). The proportions of metaphase\u2010II (M\u2010II) oocytes at the end of the 2nd culture did not differ between the FSH (75.7%) and IBMX (68.2%) groups, whereas only 10.1% of oocytes in the BM group reached the M\u2010II stage. During the 1st culture, the cAMP content of COCs and oocytes became significantly higher in the FSH and IBMX groups than in the BM group; the FSH group had a far greater increment than did the IBMX group. GJCs in the FSH and BM groups gradually closed with increasing duration of the 1st culture, whereas a significantly higher proportion of COCs in the IBMX group still had open GJCs than in the other two groups. Furthermore, LH\u2010R mRNA expression significantly increased in both the FSH and IBMX groups compared with the BM group. These results suggest that inhibition of PDEs in porcine COCs make the oocyte ready for release from meiotic arrest, and that maintenance of a moderate cAMP content may prolong GJCs and stimulate LH\u2010R expression. Mol. Reprod. Dev. 75: 857\u2013866, 2008. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20820","type":"journal-article","created":{"date-parts":[[2007,11,16]],"date-time":"2007-11-16T18:46:58Z","timestamp":1195238818000},"page":"857-866","source":"Crossref","is-referenced-by-count":27,"title":["Comparison between effects of 3\u2010isobutyl\u20101\u2010methylxanthine and FSH on gap junctional communication, LH\u2010receptor expression, and meiotic maturation of cumulus\u2013oocyte complexes in 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