{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T21:15:08Z","timestamp":1720559708425},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,11,21]],"date-time":"2007-11-21T00:00:00Z","timestamp":1195603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In embryos derived by nuclear\u2010transfer (NT), fusion of donor cells with recipient oocytes resulted in varying patterns of mitochondrial DNA (mtDNA) transmission in NT animals. Distribution of donor cell mtDNA (D\u2010mtDNA) found in offspring of NT\u2010derived founders may also vary from donor cell and host embryo heteroplasmy to host embryo homoplasmy. Here we examined the transmission of mtDNA from NT cows to G<jats:sub>1<\/jats:sub> offspring. Eleven NT founder cows were produced by fusion of enucleated oocytes (Holstein\/Japanese Black) with Jersey\/ Holstein oviduct epithelial cells, or Holstein\/Japanese Black cumulus cells. Transmission of mtDNA was analyzed by PCR mediated single\u2010strand conformation polymorphism of the D\u2010loop region. In six of seven animals sampled postmortem, heteroplasmy were detected in various tissues, while D\u2010mtDNA could not be detected in blood or hair samples from four live animals. The average proportion of D\u2010mtDNA detected in one NT cow was 7.6%, and those in other cows were &lt;5%. Heteroplasmic NT cows (n\u2009=\u20096) generated a total 12 G<jats:sub>1<\/jats:sub> offspring. Four of 12 G<jats:sub>1<\/jats:sub> offspring exhibited high percentages of D\u2010mtDNA populations (range 17\u201351%). The remaining eight G<jats:sub>1<\/jats:sub> offspring had slightly or undetectable D\u2010mtDNA (&lt;5%). Generally, a genetic bottleneck in the female germ\u2010line should favor a homoplasmic state. However, proportions of some G<jats:sub>1<\/jats:sub> offspring maintained heteroplasmy with a much higher percentage of D\u2010mtDNA than their NT dams, which may also reflect a segregation distortion caused by the proposed mitochondrial bottleneck. These results demonstrate that D\u2010mtDNA in NT cows is transmitted to G<jats:sub>1<\/jats:sub> offspring with varying efficiencies. Mol. Reprod. Dev. 75: 759\u2013765, 2008. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20837","type":"journal-article","created":{"date-parts":[[2007,11,21]],"date-time":"2007-11-21T14:46:33Z","timestamp":1195656393000},"page":"759-765","source":"Crossref","is-referenced-by-count":10,"title":["Characterization of a donor mitochondrial DNA transmission bottleneck in nuclear transfer derived cow 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