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In this study the isolated <jats:sup>32<\/jats:sup>P\u2010labelled MPS has been incorporated into mature caprine (<jats:italic>Capra indicus<\/jats:italic>) cauda\u2010epididymal spermatozoa with the help of cell electroporation technique to investigate the effect of MPS on sperm flagellar motility. The optimum conditions for electroporation of sperm cells consisted of exposure of 0.2 ml of sperm cells (2\u2009\u00d7\u200910<jats:sup>8<\/jats:sup>\/ml) to external electric field of intensity 1.5 kV\/cm and capacitation of 25 \u00b5F at 4\u00b0C and post\u2010pulse incubation at 37\u00b0C for 1 hr. when nearly 50% of the cells lost motility. Scanning electron micrographs (SEM) demonstrate the formation of micro\u2010pores and local osmotic swelling in the electroporated spermatozoa. MPS incorporation was maximal when its concentration was 30 \u00b5g\/ml (300 pmol) in the medium and when the post\u2010pulse incubation time was 60 min. At maximum (75%) MPS incorporation, total and forward motility increments were also maximum: 34% (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) and 32% (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), respectively. The subcellular fractionation data show that major portion of the introduced MPS was bound to the plasma\u2010membrane of spermatozoa. The <jats:sup>32<\/jats:sup>P\u2010labelled electroporesed intact spermatozoa lost radioactivity due to the action of the endogenous ecto\u2010phosphoprotein phosphatase. Therefore MPS is primarily localised on the sperm external surface leaving its phosphate group(s) oriented in the extracellular medium. The data provided further evidence to strengthen the view that MPS is an ecto\u2010phosphoprotein and that it plays an important role in the regulation of sperm flagellar motility. Mol. Reprod. Dev. 75: 1185\u20131195, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20854","type":"journal-article","created":{"date-parts":[[2008,1,14]],"date-time":"2008-01-14T17:53:19Z","timestamp":1200333199000},"page":"1185-1195","source":"Crossref","is-referenced-by-count":4,"title":["Role of the major ecto\u2010phosphoprotein in sperm flagellar motility using a cell electroporation 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