{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:04:55Z","timestamp":1761743095590},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,5,5]],"date-time":"2008-05-05T00:00:00Z","timestamp":1209945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Maternal recognition of pregnancy in pigs occurs approximately on Day (D) 12 of pregnancy and is critical for embryo implantation. The presence of the conceptus in the uterine lumen during this period changes uterine endometrial function to prepare for attachment of the conceptus to the endometrial epithelial cells and maintain luteal function in the ovary. Although much is known about endometrial gene expression, the genes expressed in the uterine endometria and the cellular and molecular mechanisms of those gene products during the period of implantation and maternal recognition of pregnancy in pigs are still not completely defined. To better understand the interactions between the maternal uterus and conceptus during the implantation process, we searched genes differentially expressed in the endometria on D12 of pregnancy compared to those on D12 of the estrous cycle. A new reverse transcription\u2010polymerase chain reaction (RT\u2010PCR)\u2010based method that involves annealing control primers (ACPs) was employed. Using 120 ACPs, we sequenced 12 differentially expressed genes (DEGs) and identified those genes using the Basic Local Alignment Search Tool (BLAST). Northern blot hybridization analysis confirmed the differential expression of those DEGs in the uterine endometrium. In situ hybridization analysis determined the cell\u2010type specific expression of the DEGs in the uterine endometrium. Further analysis of the DEGs found in this study will provide insights into the cellular and molecular basis of maternal and fetal interactions during the period of maternal recognition of pregnancy in the pig. Mol. Reprod. Dev. 76: 75\u201384, 2009. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20935","type":"journal-article","created":{"date-parts":[[2008,5,5]],"date-time":"2008-05-05T19:46:19Z","timestamp":1210016779000},"page":"75-84","source":"Crossref","is-referenced-by-count":22,"title":["Identification of differentially expressed genes in the uterine endometrium on day 12 of the estrous cycle and pregnancy in pigs"],"prefix":"10.1002","volume":"76","author":[{"given":"Hakhyun","family":"Ka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heewon","family":"Seo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingoo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohan","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang\u2010Kyu","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,5,5]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"677","article-title":"Different transcriptional expression of KIAA1324 and its splicing variants in human carcinoma cell lines with different metastatic capacity","volume":"11","author":"Bauer M","year":"2004","journal-title":"Oncol Rep"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-1804-3_1"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(77)90185-X"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.061110"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00157.2004"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod55.5.1098"},{"key":"e_1_2_1_8_1","first-page":"10","article-title":"A simplified in situ hybridization protocol using non\u2010radioactively labeled probes to detect abundant and rare mRNAs on tissue sections","volume":"1","author":"Braissant O","year":"1998","journal-title":"Biochemica"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700946"},{"key":"e_1_2_1_10_1","first-page":"151","article-title":"Extracellular matrix and the implantation cascade in pigs","volume":"52","author":"Burghardt RC","year":"1997","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod40.5.1109"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-1189"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.22719.x"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3180001"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0930475"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod27.4.925"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod27.4.957"},{"key":"e_1_2_1_18_1","first-page":"133","article-title":"Regulation of conceptus development and attachment in pigs","volume":"52","author":"Geisert RD","year":"1997","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_1_19_1","first-page":"163","article-title":"The use of microarrays to define functionally\u2010related genes that are differentially expressed in the cycling pig uterus","volume":"62","author":"Green JA","year":"2006","journal-title":"Soc Reprod Fertil Suppl"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-127-2-637"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00003.2004"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.2144\/03356st03","article-title":"Annealing control primer system for improving specificity of PCR amplification","volume":"35","author":"Hwang IT","year":"2003","journal-title":"Biotechniques"},{"key":"e_1_2_1_23_1","first-page":"191","article-title":"Functional analysis of autocrine and paracrine signalling at the uterine\u2013conceptus interface in pigs","volume":"58","author":"Jaeger LA","year":"2001","journal-title":"Reprod Suppl"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.3.549"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.07.008"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.6.1772"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod46.6.1165"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.2144\/04363ST02"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00434.2005"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(99)80012-X"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/323455a0"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0910469"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90326-0"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00666.2006"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830201119"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M511512200"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2520563.x"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0800133"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080420303"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00510.2006"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1673"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/245045a0"},{"key":"e_1_2_1_43_1","first-page":"53","volume-title":"Embryonic mortality in domestic species","author":"Pope WF","year":"1994"},{"key":"e_1_2_1_44_1","first-page":"6","article-title":"Microarray analysis reveals distinct gene expression profiles among different histologic types of endometrial cancer","volume":"63","author":"Risinger JI","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0820875"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(01)00087-0"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod12.1.41"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.140.9.4070"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.47.33179"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(96)00975-8"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.019307"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.037952"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90369-0"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2007.00587.x"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-0148fje"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20019"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.20935","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.20935","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T23:23:11Z","timestamp":1699917791000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.20935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5,5]]},"references-count":55,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.1002\/mrd.20935"],"URL":"https:\/\/doi.org\/10.1002\/mrd.20935","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5,5]]}}}