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It is unclear whether the sperm variation among different males would affect the outcome of oocyte evaluation by embryo development following fertilization. In the present study, variation in the developmental potential of mouse embryos was first compared between in vitro fertilization with epididymal spermatozoa from different males and Sr<jats:sup>2+<\/jats:sup> parthenogenetic activation using oocytes of different qualities, and then the effect of male on fertilization and embryo development was examined using randomly chosen oocytes and spermatozoa from cauda epididymidis, vas deferens or electro\u2010ejaculates. Rates of fertilization and blastocyst formation were significantly higher with spermatozoa from cauda epididymidis or vas deferens than with ejaculated spermatozoa. Rates of embryonic development differed significantly between different males, but not between different ejaculates of the same male. Analysis of standard errors of means and coefficients of variance indicated that as long as multiple males were involved, the variation in oocyte fertilization\/activation and blastocyst formation was always higher after fertilization than after Sr<jats:sup>2+<\/jats:sup> parthenogenetic activation whether spermatozoa were collected from epididymidis, vas deferens or ejaculates and regardless of oocyte qualities. It is concluded that (1) epididymal mouse spermatozoa fertilize more oocytes than ejaculated spermatozoa under identical experimental conditions; (2) like farm animals, the mice also show a remarkable male effect on the developmental potential of in vitro produced embryos although they are supposed to be less genetically diverse; (3) parthenogenetic activation is recommended for assessment of oocyte quality to exclude the effect of male. Mol. Reprod. Dev. 76: 239\u2013245, 2009. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20948","type":"journal-article","created":{"date-parts":[[2008,7,14]],"date-time":"2008-07-14T21:12:16Z","timestamp":1216069936000},"page":"239-245","source":"Crossref","is-referenced-by-count":6,"title":["Fertilization in vitro with spermatozoa from different mice increased variation in the developmental potential of embryos compared to artificial parthenogenetic activation"],"prefix":"10.1002","volume":"76","author":[{"given":"De\u2010Qiang","family":"Miao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun\u2010Zuo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui\u2010Li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010He","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,7,14]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"89","article-title":"In vitro embryo production from oocytes from ovaries of a single slaughtered cow","volume":"98","author":"Berg U","year":"1991","journal-title":"Dtsch Tierarztl Wochenschr"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00453-2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.5271\/sjweh.1060"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.105.18.420"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0860679"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.04.002"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00482-4"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2006000500003"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(89)90599-2"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(91)90471-O"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd1977.36.110"},{"key":"e_1_2_1_13_1","unstructured":"HoganB CostantiniF LacyE.1986.\u201cManipulating the Mouse embryo: A Laboratory Manual\u201d Cold Spring Harbor Laboratory."},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/89.3.238"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1040347"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241517598"},{"key":"e_1_2_1_17_1","unstructured":"LacalandraGM Dell AguilaME FuscoS SciorciRL PerroneP MinoiaP.1993.In vitro fertility test of bull semen. 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