{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:15:42Z","timestamp":1791018942096,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,5]],"date-time":"2009-02-05T00:00:00Z","timestamp":1233792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Dicer<\/jats:italic> encodes a riboendonuclease required for microRNA biosynthesis. <jats:italic>Dicer<\/jats:italic> was inactivated in M\u00fcllerian duct mesenchyme\u2010derived tissues of the reproductive tract of the mouse, using an <jats:italic>Amhr2\u2010Cre<\/jats:italic> allele. Although <jats:italic>Amhr2\u2010Cre<\/jats:italic>; <jats:italic>Dicer<\/jats:italic> conditional mutant males appeared normal and were fertile, mutant females were infertile. In adult mutant females, there was a reduction in the size of the oviducts and uterine horns. The oviducts were less coiled compared to controls and cysts formed at the isthmus near the uterotubal junction. Unfertilized, degenerate oocytes were commonly found within these cysts, indicating a defect in embryo transit. Beads transferred into the mutant oviduct failed to migrate into the uterus. In addition, blastocysts transferred directly into the mutant uterus did not result in pregnancy. Histological analysis demonstrated that the mutant uterus contained less glandular tissue and often the few glands that remained were found within the myometrium, an abnormal condition known as adenomyosis. In adult mutants, there was ectopic expression of <jats:italic>Wnt4<\/jats:italic> and <jats:italic>Wnt5a<\/jats:italic> in the luminal epithelium (LE) and glandular epithelium (GE) of the uterus, and <jats:italic>Wnt11<\/jats:italic> was ectopically expressed in GE. These results demonstrate that <jats:italic>Dicer<\/jats:italic> is necessary for postnatal differentiation of M\u00fcllerian duct mesenchyme\u2010derived tissues of the female reproductive tract, suggesting that microRNAs are important regulators of female reproductive tract development and fertility. Mol. Reprod. Dev. 76: 678\u2013688, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.21010","type":"journal-article","created":{"date-parts":[[2009,2,7]],"date-time":"2009-02-07T02:49:44Z","timestamp":1233974984000},"page":"678-688","source":"Crossref","is-referenced-by-count":135,"title":["<i>Dicer<\/i> is required for female reproductive tract development and fertility in the mouse"],"prefix":"10.1002","volume":"76","author":[{"given":"Gabriel","family":"Gonzalez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard 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