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Because of the close attachment of the equine oocyte to the follicle wall, scraping of the follicle is the most effective method for oocyte recovery. A notable feature of equine oocytes is that those with expanded cumuli (Ex oocytes), which originate from atretic follicles, have higher meiotic competence (ability to mature to metaphase II in vitro) than do oocytes with compact cumuli (Cp oocytes). Cp oocytes originate in viable follicles but are largely juvenile. Recovery and culture of equine oocytes immediately after slaughter yields a higher maturation rate than that obtained from oocytes after ovary storage; this is related to damage to chromatin in Cp oocytes during storage. In contrast, developmental competence (rate of blastocyst development in vitro) is higher in oocytes recovered from the ovary after a delay. The optimum duration of maturation varies based on cumulus morphology and time of recovery from the ovary, but there is no difference in developmental competence between Ex and Cp oocytes. Because standard in vitro fertilization is not repeatable in the horse, oocyte transfer (surgical transfer of oocytes to the oviducts of inseminated mares) has been developed to allow fertilization of isolated oocytes. Fertilization in vitro may be achieved using intracytoplasmic sperm injection; culture of injected oocytes in a medium with high glucose can yield over 30% blastocyst development. Mol. Reprod. Dev. 77: 651\u2013661, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.21186","type":"journal-article","created":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T09:56:28Z","timestamp":1272707788000},"page":"651-661","source":"Crossref","is-referenced-by-count":65,"title":["The equine oocyte: Factors affecting meiotic and developmental competence"],"prefix":"10.1002","volume":"77","author":[{"given":"Katrin","family":"Hinrichs","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,4,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1070215"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD08210"},{"key":"e_1_2_6_4_1","first-page":"387","article-title":"Embryo transport through the mare's oviduct depends upon cleavage and is independent of the ipsilateral corpus luteum","volume":"27","author":"Betteridge 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