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However, detailed knowledge of protein expression during somatic cell nuclear transfer (SCNT) in cattle is lacking. In the present study, variations in mitochondrial protein levels between SCNT\u2010derived and control cattle, and from calves derived by artificial insemination were investigated. Mitochondrial fractions were prepared from frozen liver samples and subjected to two\u2010dimensional (2\u2010D) fluorescence differential gel electrophoresis (DIGE) using CyDye\u2122 dyes. Protein expression changes were confirmed with a volume ratio greater than 2.0 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). 2D\u2010DIGE analysis revealed differential expression of three proteins for SCNT cattle (n\u2009=\u20094) and seven proteins for SCNT calves (n\u2009=\u20096) compared to controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Different protein patterning was observed among SCNT animals even if animals were generated from the same donor cell source. No differences were detected in two of the SCNT cattle. Moreover, there was no novel protein identified in any of the SCNT cattle or calves. In conclusion, variation in mitochondrial protein expression concentrations was observed in non\u2010viable, neonatal SCNT calves and among SCNT individuals. This result implicates mitochondrial\u2010related gene expression in early developmental loss of SCNT embryos. Comparative proteomic analysis represents an important tool for further studies on SCNT animals. Mol. Reprod. Dev. 78:263\u2013273, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.21298","type":"journal-article","created":{"date-parts":[[2011,2,21]],"date-time":"2011-02-21T21:12:19Z","timestamp":1298322739000},"page":"263-273","source":"Crossref","is-referenced-by-count":4,"title":["Comparison of liver mitochondrial proteins derived from newborn cloned calves and from cloned adult cattle by two\u2010dimensional differential gel 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