{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T23:13:25Z","timestamp":1778800405902,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2011,7,8]],"date-time":"2011-07-08T00:00:00Z","timestamp":1310083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mammalian sperm flagella have filament\u2010forming Tektin proteins (Tektin 1\u20135) reported to be involved in the stability and structural complexity of flagella. Male mice null for <jats:italic>Tektin3<\/jats:italic> produce spermatozoa with reduced forward progression and increased flagellar structural bending defects. The subcellular localization of Tektin3 (TEKT3) in spermatozoa, however, has not been clarified at the ultrastructural level. To elucidate the molecular localization of TEKT3 in flagella of rat spermatozoa, we performed extraction studies followed by immunoblot analysis, immunofluorescence microscopy, and immunogold electron microscopy. Extraction of sperm flagella from the cauda epididymis resulted in complete removal of axonemal tubulins, while TEKT3 was resistant to extraction with the same S\u2010EDTA (1% SDS, 75\u2009mM NaCl, 24\u2009mM EDTA, pH 7.6) solution, suggesting that TEKT3 might be present in the peri\u2010axonemal component and not directly associated with axonemal tubulins. Resistance to S\u2010EDTA extraction might be due to disulfide bond formation during epididymal maturation since concentrations of DTT greater than 5\u2009mM drastically promoted release of TEKT3 from flagella. Immunofluorescence microscopy and pre\u2010embedding immunoelectron microscopy revealed that TEKT3 was predominantly associated with the surface of mitochondria and outer dense fibers in the middle piece. In addition, TEKT3 was found to be present at the equatorial segment region of the acrosome membrane in sperm heads. TEKT3 might not only work as a flagellar constituent required for flagellar stability and sperm motility but also may be involved in acrosome\u2010related events, such as the acrosome reaction or sperm\u2013egg fusion. Mol. Reprod. Dev. 78:611\u2013620, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.21352","type":"journal-article","created":{"date-parts":[[2011,6,18]],"date-time":"2011-06-18T00:40:08Z","timestamp":1308357608000},"page":"611-620","source":"Crossref","is-referenced-by-count":26,"title":["Characterization and subcellular localization of Tektin 3 in rat 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