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We have generated an immortalized endothelial cell line, G\u20101410, using Simian virus 40 T\u2010antigen (SV40 T\u2010ag) primarily to overcome the short life span before the onset of senescence and high variability among enzymatically isolated cells of primary cultures. Fast proliferating cells were selected from cultures and, after a fifth passage, examined for the presence of the SV40 T\u2010ag by PCR and immunocytochemistry. Phase contrast and transmission electron microscopy revealed that G\u20101410 cells did not differ morphologically from SUVECs. The G\u20101410 cells exhibited positive staining for vascular endothelial (VE)\u2010cadherin and von Willebrand factor (vWF), and formed capillary\u2010like tube structures on Matrigel. Despite the strong oncogenic signal provided by SV40 T\u2010ag, these transformed G\u20101410 cells have remained karyotypically normal and non\u2010tumorigenic. G\u20101410 cells also responded to stimulation with VEGF, FGF\u20102, and newborn calf serum. Moreover, G\u20101410 cells showed elevated expression of VEGF120, VEGF164 (VEGF\u2010A), and FGF\u20102 at both mRNA and protein levels. In conclusion, based on the cytological and functional evaluation of the newly obtained immortalized cell line, it can be concluded that G\u20101410 cells provide a useful tool for studying the effects of VEGF and FGF systems, and other signal transduction pathways related to angiogenesis. Mol. Reprod. Dev. 78:597\u2013610, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.21353","type":"journal-article","created":{"date-parts":[[2011,6,18]],"date-time":"2011-06-18T00:36:56Z","timestamp":1308357416000},"page":"597-610","source":"Crossref","is-referenced-by-count":12,"title":["Immortalization of swine umbilical vein endothelial cells (SUVECs) with the simian virus 40 large\u2010T antigen"],"prefix":"10.1002","volume":"78","author":[{"given":"Marcin","family":"Chrusciel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Bodek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lech","family":"Kirtiklis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bogdan","family":"Lewczuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire L.","family":"Hyder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Blitek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika M.","family":"Kaczmarek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam J.","family":"Ziecik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aneta","family":"Andronowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,7,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209046"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.2000.0342"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.2005.B0631"},{"key":"e_1_2_7_5_1","first-page":"479","article-title":"The expression of short form of leptin receptor gene during early pregnancy in the pig examined by quantitative real time RT\u2010PCR","volume":"57","author":"Bogacka I","year":"2006","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012259529167"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevOncog.v5.i4.10"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20494"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00213"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205382"},{"key":"e_1_2_7_12_1","unstructured":"Cell Bank RIKEN BioResource Center database. 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