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In the lizard <jats:italic>Podarcis sicula<\/jats:italic>, the epididymis can be regionalized to an initial segment called the <jats:italic>caput<\/jats:italic> that comprises the <jats:italic>efferent ductules<\/jats:italic>, followed by the middle and terminal segments, respectively termed the <jats:italic>corpus<\/jats:italic> and <jats:italic>cauda<\/jats:italic>. By means of in situ hybridization and immunocytochemistry, we analyzed the expression of the estrogen receptors of the alpha and beta type (ER\u03b1 and ER\u03b2) in Podarcis to test the responsiveness of the epididymal regions to estrogen in the annual reproductive cycle of this seasonal breeder. The results show that the <jats:italic>efferent ductules<\/jats:italic> and the <jats:italic>cauda<\/jats:italic> always express both ER\u03b1 and ER\u03b2 throughout the year. In the <jats:italic>corpus<\/jats:italic>, the expression of ER\u03b1 takes place only at the end of the mating period and continues in the non\u2010reproductive season whereas ER\u03b2 is expressed in all phases of the cycle. During the mating season, the cells of the <jats:italic>corpus<\/jats:italic> are engaged in massive secretory activity and do not express ER\u03b1. Experimental administration of E<jats:sub>2<\/jats:sub> during this season does not change the expression of ER\u03b2, nor does it affect the <jats:italic>efferent ductules<\/jats:italic> and <jats:italic>cauda<\/jats:italic>; instead, it inhibits the secretory activity in the <jats:italic>corpus<\/jats:italic> and induces the expression of ER\u03b1. Taken together, our findings suggest that in the epididymis of Podarcis, the expression of ER\u03b1 may act as a switch for the secretory activity of the epididymal <jats:italic>corpus<\/jats:italic>. Mol. Reprod. Dev. 79:107\u2013117, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/mrd.22005","type":"journal-article","created":{"date-parts":[[2011,11,18]],"date-time":"2011-11-18T20:18:23Z","timestamp":1321647503000},"page":"107-117","source":"Crossref","is-referenced-by-count":14,"title":["Expression of estrogen receptor alpha switches off secretory activity in the epididymal channel of the lizard <i>Podarcis sicula<\/i>"],"prefix":"10.1002","volume":"79","author":[{"given":"Mariailaria","family":"Verderame","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Angelini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ermelinda","family":"Limatola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,12,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-6480(92)90165-G"},{"key":"e_1_2_7_3_1","unstructured":"AngeliniF BotteV.1992.Spermatogenesis in reptiles dynamic and regulatory aspect. 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