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Herein we document this phenomenon in porcine in vitro\u2010fertilized (IVF), somatic cell nuclear transfer (SCNT), and parthenogenetic (PA) embryos. In vitro\u2010matured pig oocytes were used to generate IVF, SCNT, and PA embryos. At 24\u2009hr post\u2010activation (or insemination; hpa\/hpi), embryos were visually assessed, and cleaved embryos were moved into a new culture well. This process was repeated at 30 and 48 hpa\/hpi. All embryos were allowed to develop 7 days in culture. For IVF embryos, 39.9%, 24.6%, and 10.5% of fast\u2010, intermediate\u2010, or slow\u2010cleaving embryos, respectively, developed into blastocysts by day 7. For SCNT embryos, 31.8% of fast\u2010, 5.7% of intermediate\u2010, and 2.9% of late\u2010cleaving embryos achieved the blastocyst stage of development. For PA embryos, the percentages of those cleaved embryos that developed to blastocyst were 59.3%, 36.7%, and 7.5% for early\u2010, intermediate\u2010, and late\u2010cleaving embryos, respectively. Using RNA collected from early\u2010, intermediate\u2010, and late\u2010cleaving embryos, real\u2010time PCR was performed to assess the transcript levels of 14 different genes of widely varied function. The qPCR results suggest that maternal mRNA degradation may not proceed in an appropriate pattern in slow\u2010cleaving embryos. These findings (1) confirm that, as observed in other species, earlier\u2010cleaving porcine embryos are more successful at developing in culture than are slower\u2010cleaving embryos, and (2) implicate mechanisms of maternal transcript destruction as potential determinants of oocyte\/embryo quality. Mol. Reprod. Dev. 79: 197\u2013207, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/mrd.22013","type":"journal-article","created":{"date-parts":[[2011,12,3]],"date-time":"2011-12-03T17:30:36Z","timestamp":1322933436000},"page":"197-207","source":"Crossref","is-referenced-by-count":55,"title":["Timing of first embryonic cleavage is a positive indicator of the in vitro developmental potential of porcine embryos derived from in vitro fertilization, somatic cell nuclear transfer and parthenogenesis"],"prefix":"10.1002","volume":"79","author":[{"given":"S. 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