{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:47:31Z","timestamp":1788871651740,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,9,14]],"date-time":"2012-09-14T00:00:00Z","timestamp":1347580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["WO 663\/7-1"],"award-info":[{"award-number":["WO 663\/7-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Recently, we identified an N\u2010terminally truncated form of the mRNA cap binding protein eIF4E in the porcine luminal epithelium during implantation. EIF4E truncation is accompanied by degradation of the eIF4E\u2010repressor protein 4E\u2010BP1. In this study, we investigated whether or not the other members of the eIF4E\u2010repressor family, namely 4E\u2010BP2 and 4E\u2010BP3, were also modified during early pregnancy. We did not detect 4E\u2010BP3 in the uterine tissue; however, 4E\u2010BP2 emerged in one or two stable fragments on pregnancy day 15. 4E\u2010BP2 truncation most likely occurs at the N\u2010terminus, and this calcium\u2010stimulated processing depends on progesterone and estradiol. The activities targeting eIF4E, 4E\u2010BP1, and 4E\u2010BP2 were found in different fractions after anionic exchange chromatography, indicating the action of different proteases. Detailed protein interaction studies with immobilized anti\u2010eIF4E and m\n                    <jats:sup>7<\/jats:sup>\n                    GTP\u2010Sepharose showed a differential binding of the 4E\u2010BP2 isoforms to the eIF4E variants and to the cap structure. In general, truncation of eIF4E reduces the inhibitory impact of 4E\u2010BP2, whereas truncation of 4E\u2010BP2 restores repression by binding the prototype eIF4E. In this context, we suggest long\u2010term translational repression by the truncated 4E\u2010BP2 is affected by the loss of the RAIP motif located at the N\u2010terminus, which is indispensable for phosphorylation and deactivation of the molecule. In conclusion, we propose a tightly balanced regulation of the truncation of the cap\u2010binding complex component eIF4F and degradation of 4E\u2010BP1 and\/or truncation of 4E\u2010BP2 that together ensures correct translational control during the dynamic process of conceptus implantation. Mol. Reprod. Dev. 79: 767\u2013776, 2012. \u00a9 2012 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/mrd.22108","type":"journal-article","created":{"date-parts":[[2012,9,11]],"date-time":"2012-09-11T21:38:27Z","timestamp":1347399507000},"page":"767-776","source":"Crossref","is-referenced-by-count":4,"title":["The eIF4E repressor protein 4E\u2010BP2 is merely truncated, despite 4E\u2010BP1 degradation in the porcine uterine tissue during implantation"],"prefix":"10.1002","volume":"79","author":[{"given":"Karin","family":"Wollenhaupt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus\u2010Peter","family":"Br\u00fcssow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Albrecht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Tomek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2423-05.2005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5196-05.2006"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nlm.2006.08.012"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.160.7.3269"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308573200"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/clo.2006.8.259"},{"key":"e_1_2_7_8_1","first-page":"33","volume-title":"Textbook 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