{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:34:01Z","timestamp":1790940841235,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,4,19]],"date-time":"2013-04-19T00:00:00Z","timestamp":1366329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grant-in-Aid for Scientific Research","award":["23780279"],"award-info":[{"award-number":["23780279"]}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Promotion of Basic Research Activities for Innovative Bioscience"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Interferon tau (IFNT), produced for a short interval during early pregnancy by the ruminant embryonic trophectoderm, is essential for the maintenance of early pregnancy, but the mechanism by which it is transcriptionally regulated has not been fully determined. To identify a transcription factor(s) involved in the down\u2010regulation of <jats:italic>IFNT<\/jats:italic> genes, mRNAs for various known transcription factors were investigated by reverse\u2010transcriptase and real\u2010time PCR in conceptus tissues collected on Days 15, 17, and 21, or Days 17, 20, and 22 of ovine or bovine pregnancy, respectively. In particular, the T\u2010box protein eomesodermin (<jats:italic>EOMES<\/jats:italic>) exhibited high mRNA expression in Day 17 or 22 ovine or bovine conceptuses. Interaction between EOMES and the identified transcription factors was studied using transient transfection, revealing that ovine\/bovine <jats:italic>IFNT<\/jats:italic>\u2010reporter transactivation was down\u2010regulated by EOMES. Transcription factor interactions with EOMES were further studied through immunoprecipitation, demonstrating an association between EOMES and cAMP\u2010response element binding protein (CREB)\u2010binding protein (CREBBP). Uterine flushing media collected from cyclic or early pregnancy animals were added to bovine trophoblast CT\u20101 cells cultured on type\u2010I collagen (monoculture) or bovine uterine epithelial cells (coculture) in an attempt to regulate <jats:italic>EOMES<\/jats:italic> expression. In the coculture, but not the monoculture, addition of uterine flushing from Day 17 pregnant animals resulted in increased <jats:italic>EOMES<\/jats:italic> expression in CT\u20101 cells. These results suggest that as conceptuses attach to the uterine epithelium, <jats:italic>IFNT<\/jats:italic> gene transcription is down\u2010regulated by an increase in EOMES expression and EOMES\u2013CREBBP binding in the attached trophoblast cells. <jats:italic>Mol. Reprod. Dev. 80: 371\u2013383, 2013. \u00a9 2013 Wiley Periodicals, Inc<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22171","type":"journal-article","created":{"date-parts":[[2013,4,19]],"date-time":"2013-04-19T17:38:54Z","timestamp":1366393134000},"page":"371-383","source":"Crossref","is-referenced-by-count":23,"title":["Down\u2010regulation of interferon tau gene transcription with a transcription factor, EOMES"],"prefix":"10.1002","volume":"80","author":[{"given":"Toshihiro","family":"Sakurai","sequence":"first","affiliation":[{"name":"Laboratory of Animal Breeding and Reproduction Graduate School of Agricultural and Life Sciences, The University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanako","family":"Bai","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Breeding and Reproduction Graduate School of Agricultural and Life Sciences, The University of Tokyo Tokyo 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