{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:55:10Z","timestamp":1790942110156,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,8,19]],"date-time":"2013-08-19T00:00:00Z","timestamp":1376870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"funder":[{"name":"NIH","award":["R01HD051999"],"award-info":[{"award-number":["R01HD051999"]}]},{"name":"NIH","award":["R01HD057144"],"award-info":[{"award-number":["R01HD057144"]}]},{"name":"NIH","award":["T32HD007305"],"award-info":[{"award-number":["T32HD007305"]}]},{"name":"NIH","award":["P30ES013508"],"award-info":[{"award-number":["P30ES013508"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:label\/><jats:p>Triosephosphate isomerase 1 (TPI1) is a member of the glycolytic pathway, which is a critical source of energy for motility in mouse sperm. By immunoblotting, we detected two male, germ line\u2010specific TPI1 bands (M<jats:sub>r<\/jats:sub> 33,400 and 30,800) as well as the somatic\u2010type band (M<jats:sub>r<\/jats:sub> 27,700). Although all three bands were observed in spermatogenic cells, somatic\u2010type TPI1 disappeared from sperm during epididymal maturation. In vitro dephosphorylation analysis suggested that the two male, germ line\u2010specific TPI1 bands were not the result of phosphorylation of the 27,700 M<jats:sub>r<\/jats:sub> TPI1 band. The M<jats:sub>r<\/jats:sub> 33,400; 30,800; and 27,700 TPI1 bands corresponded to the respective sizes of the proteins predicted to use the first, second, and third possible initiation codons of the <jats:italic>Tpi1<\/jats:italic> cDNA. We performed immunofluorescence on epididymal sperm and determined that TPI1 specifically localized in the principal piece. The antibody staining was stronger in cauda epididymal sperm than in caput epididymal sperm, a finding consistent with the identification of TPI1 as a cauda epididymal sperm\u2010enriched protein. Immunofluorescence with sodium dodecyl sulfate (SDS)\u2010insoluble flagellar accessory structures showed a strong TPI1 signal only in the principal piece, indicating that TPI1 is a component of the fibrous sheath. Northern blot hybridization detected longer <jats:italic>Tpi1<\/jats:italic> transcripts (1.56\u2009kb) in mouse testis, whereas somatic tissues had shorter transcripts (1.32\u2009kb). As there is only one triosephosphate isomerase gene in the mouse genome, we conclude that the three variants we see in sperm result from the use of alternative translation start codons in spermatogenic cells. <jats:italic>Mol. Reprod. Dev. 80: 862\u2013870, 2013. \u00a9 2013 The Authors<\/jats:italic>. Published by Wiley Periodicals, Inc. This is an open access article under the terms of the <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/\">Creative Commons Attribution\u2010NonCommercial\u2010NoDerivs<\/jats:ext-link> License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non\u2010commercial and no modifications or adaptations are made.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22217","type":"journal-article","created":{"date-parts":[[2013,7,23]],"date-time":"2013-07-23T04:45:37Z","timestamp":1374554737000},"page":"862-870","source":"Crossref","is-referenced-by-count":14,"title":["Male mice express spermatogenic cell\u2010specific triosephosphate isomerase isozymes"],"prefix":"10.1002","volume":"80","author":[{"given":"Takashi W.","family":"Ijiri","sequence":"first","affiliation":[{"name":"Center for Research on Reproduction and Women's Health Perelman School of Medicine University of Pennsylvania Philadelphia Pennsylvania"},{"name":"Department of Molecular Biosciences Kyoto Sangyo University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa L.","family":"Vadnais","sequence":"additional","affiliation":[{"name":"Center for Research on Reproduction and Women's Health Perelman School of Medicine University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel M.","family":"Lin","sequence":"additional","affiliation":[{"name":"Center for Research on Reproduction and Women's Health Perelman School of Medicine University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy P.","family":"Huang","sequence":"additional","affiliation":[{"name":"Center for Research on Reproduction and Women's Health Perelman School of Medicine University of Pennsylvania Philadelphia 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