{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T07:16:39Z","timestamp":1778742999898,"version":"3.51.4"},"reference-count":52,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T00:00:00Z","timestamp":1381968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:label\/><jats:p>We determined if somatic cell nuclear transfer (SCNT) cloning is associated with WNT\u2010related gene expression in cattle development, and if the expression of genes in the WNT pathway changes during the peri\u2010implantation period. Extra\u2010embryonic and endometrial tissues were collected at gestation days 18 and 34 (d18, d34). <jats:italic>WNT5A<\/jats:italic>, <jats:italic>FZD4<\/jats:italic>, <jats:italic>FZD5<\/jats:italic>, <jats:italic>LRP5<\/jats:italic>, <jats:italic>CTNNB1<\/jats:italic>, <jats:italic>GNAI2<\/jats:italic>, <jats:italic>KDM1A<\/jats:italic>, <jats:italic>BCL2L1<\/jats:italic>, and <jats:italic>SFRP1<\/jats:italic> transcripts were localized in extra\u2010embryonic tissue, whereas <jats:italic>SFRP1<\/jats:italic> and <jats:italic>DKK1<\/jats:italic> were localized in the endometrium. There were no differences in the localization of these transcripts in extra\u2010embryonic tissue or endometrium from SCNT or artificial insemination (AI) pregnancies. Expression levels of <jats:italic>WNT5A<\/jats:italic> were 11\u2010fold greater in the allantois of SCNT than AI samples. In the trophoblast, expression of <jats:italic>WNT5A<\/jats:italic>, <jats:italic>FZD5<\/jats:italic>, <jats:italic>CTNNB1<\/jats:italic>, and <jats:italic>DKK1<\/jats:italic> increased significantly from d18 to d34, whereas expression of <jats:italic>KDM1A<\/jats:italic> and <jats:italic>SFRP1<\/jats:italic> decreased, indicating that implantation is associated with major changes in WNT signaling. SCNT was associated with altered <jats:italic>WNT5A<\/jats:italic> expression in trophoblasts, with levels increasing 2.3\u2010fold more in AI than SCNT conceptuses from d18 to d34. In the allantois, expression of <jats:italic>WNT5A<\/jats:italic> increased 6.3\u2010fold more in SCNT than AI conceptuses from d18 to d34. Endometrial tissue expression levels of the genes tested did not differ between AI or SCNT pregnancies, although expression of individual genes showed variation across developmental stages. Our results demonstrate that SCNT is associated with altered expression of specific WNT\u2010related genes in extra\u2010embryonic tissue in a time\u2010 and tissue\u2010specific manner. The pattern of gene expression in the WNT pathway suggests that noncanonical WNT signal transduction is important for implantation of cattle conceptuses. <jats:italic>Mol. Reprod. Dev. 80: 977\u2013987, 2013. \u00a9 2013 Wiley Periodicals, Inc<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22257","type":"journal-article","created":{"date-parts":[[2013,8,27]],"date-time":"2013-08-27T13:49:18Z","timestamp":1377611358000},"page":"977-987","source":"Crossref","is-referenced-by-count":16,"title":["Changes in WNT signaling\u2010related gene expression associated with development and cloning in bovine extra\u2010embryonic and endometrial tissues during the peri\u2010implantation period"],"prefix":"10.1002","volume":"80","author":[{"given":"Fernando H.","family":"Biase","sequence":"first","affiliation":[{"name":"Institute for Genomic Biology University of Illinois at Urbana\u2010Champaign Urbana Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chanaka","family":"Rabel","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences University of Illinois at Urbana\u2010Champaign Urbana 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