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Loss or gain of adhesiveness also dictates the potential of endometrial epithelial cells to metastasize, an event that can result from certain genetic insults. A proteomics\u2010based exploration of the \u201cadhesiveness\u201d these epithelial cells was employed that could identify targets that could disrupt embryo\u2013endometrium interactions and\/or metastasis of endometrial cancer cells. The present study defined the surfactomes of two human endometrial epithelial cell lines known for their differential adhesiveness to embryonic cells. Comparative, two\u2010dimensional electrophoretic analysis of the surfactomes of RL95\u20102 (exhibiting higher adhesiveness to the embryonic cell line JAr) and HEC\u20101A (exhibiting reduced adhesiveness to JAr cells) revealed 55 differentially enriched proteins. Of these, 10 proteins were identified by MALDI\u2010TOF\/TOF or LC\u2013MS\/MS. TUBB2C, ADAMTS3, and elongation factor beta were more abundant on the HEC\u20101A cell surface whereas HSP27, HSPA9, GP96, CRT, Tapasin\u2010ERP57, PDI, and \u03b2\u2010actin were more abundant on the RL95\u20102 cell surface. Nano LC\u2013MS\/MS was also employed to generate a more comprehensive surfactomes of RL95\u20102 and HEC\u20101A. The study also demonstrated a pro\u2010adhesive role of CRT and HSPA9 and an anti\u2010adhesive role of TUBB2C populations found on the cell surface. In brief, this study identifies the cell\u2010surface protein complements of two human endometrial epithelial cell lines, and reveals the role of three proteins in heterotypic cell adhesion. <jats:italic>Mol. Reprod. Dev<\/jats:italic>. 81: 326\u2013340, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22301","type":"journal-article","created":{"date-parts":[[2014,1,10]],"date-time":"2014-01-10T17:06:30Z","timestamp":1389373590000},"page":"326-340","source":"Crossref","is-referenced-by-count":16,"title":["Cell surfactomes of two endometrial epithelial cell lines that differ in their adhesiveness to embryonic cells"],"prefix":"10.1002","volume":"81","author":[{"given":"Sonali R.","family":"Bhagwat","sequence":"first","affiliation":[{"name":"Primate Biology Laboratory National Institute for Research in Reproductive Health, Indian Council of Medical Research Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tejashree","family":"Redij","sequence":"additional","affiliation":[{"name":"Primate Biology Laboratory National Institute for Research in Reproductive Health, Indian Council of Medical Research Mumbai 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