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The present work was undertaken to investigate the methylation profile of differentially methylated regions (DMRs) in the <jats:italic>IGF2\u2010H19<\/jats:italic> locus of <jats:italic>Bos taurus<\/jats:italic> X <jats:italic>Bos indicus<\/jats:italic> crossbred bull spermatozoa. Bulls having more than at least 100 insemination records over a period of 12 years were classified into two groups of five bulls each belonging to low\u2010 and high\u2010fertility groups. The <jats:italic>IGF2<\/jats:italic> and <jats:italic>H19<\/jats:italic> DMR sequences in <jats:italic>B. indicus<\/jats:italic> cattle were observed to be in absolute homology with <jats:italic>B. taurus<\/jats:italic> cattle. The DNA of crossbred bull spermatozoa was isolated, bisulfite treated, and amplified for specific DMR regions using methylation\u2010change\u2010specific primers. The overall degree of methylation at <jats:italic>IGF2\u2010H19<\/jats:italic> DMRs was not found to be significantly different among two groups of bulls. The sixth CTCF binding site (CCCTC) identified in <jats:italic>H19<\/jats:italic> DMR, however, had a significant methylation difference between the high\u2010 and low\u2010fertility bulls. It was concluded that alteration of the methylation levels at <jats:italic>IGF2\u2010H19<\/jats:italic> DMRs might not be responsible for the fertility difference of crossbred bulls, although the role played by the specific CTCF binding sites at this locus, which could influence <jats:italic>IGF2<\/jats:italic> expression during spermatogenesis and early embryonic development, deserves further attention. <jats:italic>Mol. Reprod. Dev<\/jats:italic>. 81: 350\u2013362, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22303","type":"journal-article","created":{"date-parts":[[2014,1,17]],"date-time":"2014-01-17T06:49:30Z","timestamp":1389941370000},"page":"350-362","source":"Crossref","is-referenced-by-count":20,"title":["Differential methylation status of <i>IGF2\u2010H19<\/i> locus does not affect the fertility of crossbred bulls but some of the CTCF binding sites could be potentially important"],"prefix":"10.1002","volume":"81","author":[{"given":"Subas C.","family":"Jena","sequence":"first","affiliation":[{"name":"Animal Biotechnology Centre National Dairy Research Institute Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandeep","family":"Kumar","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Centre National Dairy Research Institute Karnal Haryana 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