{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:13:11Z","timestamp":1767849191238,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,12,8]],"date-time":"2014-12-08T00:00:00Z","timestamp":1417996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Economy and Competitiveness of Spain and European Regional Development Fund","award":["AGL2012-39903"],"award-info":[{"award-number":["AGL2012-39903"]}]},{"DOI":"10.13039\/501100001862","name":"Swedish Research council Formas","doi-asserted-by":"crossref","award":["2011-512"],"award-info":[{"award-number":["2011-512"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:label\/><jats:p>This study revealed and characterised the presence of the antioxidant enzymes paraoxonase (PON) type 1 (PON\u20101, extracellular) and type 2 (PON\u20102, intracellular) in boar semen. To evaluate PON\u20101, an entire ejaculate from each of ten boars was collected and the seminal plasma was harvested after double centrifugation (1,500<jats:italic>g<\/jats:italic> for 10\u2009min). Seminal plasma was analysed for concentration as well as enzymatic activity of PON\u20101 and total cholesterol levels. Seminal\u2010plasma PON\u20101 concentration ranged from 0.961 to 1.670\u2009ng\/ml while its enzymatic activity ranged from 0.056 to 0.400 IU\/ml, which represent individual variance. Seminal\u2010plasma PON\u20101 concentration and enzymatic activity were negatively correlated (r\u2009=\u2009\u22120.763; <jats:italic>P\u2009<\/jats:italic>&lt;\u20090.01). The activity of seminal\u2010plasma PON\u20101 negatively correlated with ejaculate volume (r\u2009=\u2009\u22120.726, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), but positively correlated with sperm concentration (r\u2009=\u20090.654, <jats:italic>P\u2009<\/jats:italic>&lt;\u20090.05). Total seminal\u2010plasma cholesterol concentration positively correlated with PON\u20101 activity (r\u2009=\u20090.773; <jats:italic>P\u2009<\/jats:italic>&lt;\u20090.01), but negatively correlated with PON\u20101 concentration (r\u2009=\u2009\u22120.709; <jats:italic>P\u2009<\/jats:italic>&lt;\u20090.05). The presence of intracellular PON\u20102 was determined via immunocytochemistry in spermatozoa derived from artificial insemination. PON\u20102 localised to the post\u2010acrosomal area of the sperm head and principal piece of the tail in membrane\u2010intact spermatozoa. In summary, PON is present in boar semen, with PON\u20101 at low levels in seminal plasma and PON\u20102 within the spermatozoa. Further studies are needed to characterise the relationship between antioxidant PONs with sperm and other seminal\u2010plasma parameters. <jats:italic>Mol. Reprod. Dev. 82: 58\u201365, 2015. \u00a9 2014 Wiley Periodicals, Inc<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22444","type":"journal-article","created":{"date-parts":[[2014,12,9]],"date-time":"2014-12-09T12:21:12Z","timestamp":1418127672000},"page":"58-65","source":"Crossref","is-referenced-by-count":22,"title":["Measurement of activity and concentration of paraoxonase 1 (PON\u20101) in seminal plasma and identification of PON\u20102 in the sperm of boar ejaculates"],"prefix":"10.1002","volume":"82","author":[{"given":"Isabel","family":"Barranco","sequence":"first","affiliation":[{"name":"Department of Medicine and Animal Surgery University of Murcia Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordi","family":"Roca","sequence":"additional","affiliation":[{"name":"Department of Medicine and Animal Surgery University of Murcia Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asta","family":"Tvarijonaviciute","sequence":"additional","affiliation":[{"name":"Department of Medicine and Animal Surgery University of Murcia Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Rub\u00e9r","sequence":"additional","affiliation":[{"name":"Department of Clinical &amp; Experimental Medicine (IKE) University of Link \u00f6 ping Link\u00f6ping Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Vicente\u2010Carrillo","sequence":"additional","affiliation":[{"name":"Department of Clinical &amp; Experimental Medicine (IKE) University of Link \u00f6 ping Link\u00f6ping Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Atikuzzaman","sequence":"additional","affiliation":[{"name":"Department of Clinical &amp; Experimental Medicine (IKE) University of Link \u00f6 ping Link\u00f6ping Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose J.","family":"Ceron","sequence":"additional","affiliation":[{"name":"Department of Medicine and Animal Surgery University of Murcia Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio A.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Department of Medicine and Animal Surgery University of Murcia Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heriberto","family":"Rodriguez\u2010Martinez","sequence":"additional","affiliation":[{"name":"Department of Clinical &amp; Experimental Medicine (IKE) University of Link \u00f6 ping Link\u00f6ping Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,12,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2004.06.030"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(98)00272-X"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.076471"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2005.06.010"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2011.01826.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.07.009"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000141564.89465.4E"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408360802610878"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(99)00035-4"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","unstructured":"Ceron JJ Tecles F Tvarijonaviciute. 2014. 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