{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:22:37Z","timestamp":1767853357334,"version":"3.49.0"},"reference-count":67,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,5,10]],"date-time":"2016-05-10T00:00:00Z","timestamp":1462838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["A04\u2010M55\u201006A"],"award-info":[{"award-number":["A04\u2010M55\u201006A"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["298314\u201011"],"award-info":[{"award-number":["298314\u201011"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["155065\u201006"],"award-info":[{"award-number":["155065\u201006"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:label\/><jats:p>Seminiferous tubules of the testis and epididymal tubules in adult rodents are enveloped by contractile myoid cells, which move sperm and fluids along the male reproductive tract. Myoid cells in the testis influence Sertoli cells by paracrine signaling, but their role in the epididymis is unknown. Electron microscopy revealed that elongated myoid cells formed several concentric layers arranged in a loose configuration. The edges of some myoid cells in a given layer closely approximated one another, and extended small foot\u2010like processes to cells of overlying layers. Gap junction proteins, connexins 32 and 43, were detected within the myoid cell layers by immunohistochemistry. These myoid cells also had caveolae that contained caveolin\u20101 and cavin\u20101 (also known as PTRF). The number of caveolae per unit area of plasma membrane was significantly reduced in caveolin\u20101\u2010deficient mice (<jats:italic>Cav1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>). Morphometric analyses of <jats:italic>Cav1<\/jats:italic>\u2010null testes revealed an enlargement in whole\u2010tubule and epithelial profile areas, whereas these parameters were slightly reduced in the epididymis. Although sperm are non\u2010motile as they pass through the proximal epididymis, statistical analyses of cauda epididymidis sperm concentrations revealed no significant differences between wild\u2010type and <jats:italic>Cav1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice. Motility analyses, however, indicated that sperm velocity parameters were reduced while beat cross frequency was higher in gametes of <jats:italic>Cav1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice. Thus while caveolae and their associated proteins are not necessary for myoid cell contractility, they appear to be crucial for signaling with the epididymal epithelium to regulate the proper acquisition of sperm motility. <jats:italic>Mol. Reprod. 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