{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T22:50:01Z","timestamp":1767826201327,"version":"3.49.0"},"reference-count":66,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,6,6]],"date-time":"2016-06-06T00:00:00Z","timestamp":1465171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Council of Science and Technology","award":["CONACyT 166727"],"award-info":[{"award-number":["CONACyT 166727"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:label\/><jats:p>Cytoskeleton remodeling is necessary for capacitation and the acrosome reaction in spermatozoa. F\u2010actin is located in the acrosome and equatorial region during capacitation, but is relocated in the post\u2010acrosomal region during the acrosome reaction in spermatozoa from bull, rat, mice, and guinea pig. Actin polymerization and relocalization are generally regulated by small GTPases that activate Wasp protein, which coordinates with Arp2\/3, profilin I, and profilin II to complete cytoskeletal remodeling. This sequence of events is not completely described in spermatozoa, though. Therefore, the aim of this study was to determine if Wasp interacts with small GTPases (RhoA, RhoB, and Cdc42) and proteins (Arp2\/3, profilin I, and profilin II) that co\u2010localize with F\u2010actin during capacitation and the acrosome reaction in English guinea pig spermatozoa obtained from the vas deferens. The spermatozoa were capacitated in calcium\u2010free medium, incubated with an activator or an inhibitor of GTPases, and then induced to acrosome react using calcium. The distribution patterns of F\u2010actin were compared to the patterns of Wasp and its putative interaction partners: Wasp and RhoB, but not RhoA or Cdc42, localization overlap with F\u2010actin during capacitation and the acrosome reaction. Activation of small GTPases localized RhoB to the post\u2010acrosomal region whereas their inhibition prevented acrosome exocytosis. Arp2\/3 and profilin II appear to interact with Wasp in the post\u2010acrosomal region and flagellum, while profilin I and Wasp could be found in the equatorial region. Thus, Wasp and F\u2010actin distribution overlap during capacitation and acrosome reaction, and small GTPases play an important role in cytoskeleton remodeling during these processes in spermatozoa.<jats:italic>Mol. Reprod. Dev. 83: 927\u2013937, 2016 \u00a9 2016 Wiley Periodicals, Inc<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22657","type":"journal-article","created":{"date-parts":[[2016,5,16]],"date-time":"2016-05-16T21:53:11Z","timestamp":1463435591000},"page":"927-937","source":"Crossref","is-referenced-by-count":15,"title":["Role of Wasp and the small GTPases RhoA, RhoB, and Cdc42 during capacitation and acrosome reaction in spermatozoa of English guinea pigs"],"prefix":"10.1002","volume":"83","author":[{"given":"Norma L.","family":"Delgado\u2010Buenrostro","sequence":"first","affiliation":[{"name":"Unidad de Biomedicina UBIMED Facultad de Estudios Superiores Iztacala Universidad Nacional Aut\u00f3noma de M\u00e9xico Tlalnepantla Estado de M\u00e9xico CP 54090"},{"name":"Secci\u00f3n de Bioqu\u00edmica y Farmacolog\u00eda Humana Facultad de Estudios Superiores Cuautitl\u00e1n Universidad Nacional Aut\u00f3noma de M\u00e9xico Cuautitl\u00e1n Estado de M\u00e9xico CP 54743"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adela","family":"M\u00fajica","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional M\u00e9xico DF M\u00e9xico CP 07360"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Chiquete\u2010Felix","sequence":"additional","affiliation":[{"name":"Instituto de Fisiolog\u00eda Celular Universidad Nacional Aut\u00f3noma de M\u00e9xico M\u00e9xico DF M\u00e9xico CP 04510"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"D\u00e9ciga\u2010Alcaraz","sequence":"additional","affiliation":[{"name":"Unidad de Biomedicina UBIMED Facultad de Estudios Superiores Iztacala Universidad Nacional Aut\u00f3noma de M\u00e9xico Tlalnepantla Estado de M\u00e9xico CP 54090"},{"name":"Programa de Doctorado en Ciencias Biom\u00e9dicas Universidad Nacional Aut\u00f3noma de M\u00e9xico CP 07360"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Estefany I.","family":"Medina\u2010Reyes","sequence":"additional","affiliation":[{"name":"Unidad de Biomedicina UBIMED Facultad de Estudios Superiores Iztacala Universidad Nacional Aut\u00f3noma de M\u00e9xico Tlalnepantla Estado de M\u00e9xico CP 54090"},{"name":"Programa de Doctorado en Ciencias Biom\u00e9dicas Universidad Nacional Aut\u00f3noma de M\u00e9xico CP 07360"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvador","family":"Uribe\u2010Carvajal","sequence":"additional","affiliation":[{"name":"Instituto de Fisiolog\u00eda Celular Universidad Nacional Aut\u00f3noma de M\u00e9xico M\u00e9xico DF M\u00e9xico CP 04510"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yolanda I.","family":"Chirino","sequence":"additional","affiliation":[{"name":"Unidad de Biomedicina UBIMED Facultad de Estudios Superiores Iztacala Universidad Nacional Aut\u00f3noma de M\u00e9xico Tlalnepantla Estado de M\u00e9xico CP 54090"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,6,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.114.015036"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gat067"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-11-0433"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5AN00979K"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201502062"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.154305"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00269"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.009233"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.114.117911"},{"key":"e_1_2_7_12_1","first-page":"311","article-title":"Identification of actin in boar spermatids and spermatozoa by immunoelectron microscopy","volume":"42","author":"Camatini M","year":"1986","journal-title":"Eur J Cell Biol"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-015-1914-2"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01599"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080330114"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1512464112"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.010909.105753"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.4161\/sgtp.29469"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20192"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199406003790"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20110625"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.107.062943"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2013.06.014"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092210206"},{"key":"e_1_2_7_25_1","first-page":"1621","article-title":"Characterization of the lectin binding pattern in human spermatozoa after swim\u2010up selection","volume":"27","author":"G\u00f3mez\u2010Torres MJ","year":"2012","journal-title":"Histol Histopathol"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2012.03.008"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5350.509"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20120103"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)02976-9"},{"key":"e_1_2_7_30_1","article-title":"Lysophosphatidic acid receptors LPA4 and LPA6 differentially promote lymphocyte transmigration across high endothelial venules in lymph nodes","author":"Hata E","year":"2015","journal-title":"Int Immunol"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2476"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0169(200005)46:1<43::AID-CM5>3.0.CO;2-1"},{"key":"e_1_2_7_33_1","article-title":"Lysophosphatidic acid activates the RhoA and NF\u2010\u03baB through Akt\/I\u03baB\u03b1 signaling and promotes prostate cancer invasion and progression by enhancing functional invadopodia formation","author":"Hwang YS","year":"2015","journal-title":"Tumor Biol"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1304"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.12.002"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1999.4197"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1995.0281i.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12076"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0139094"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.4161\/21541248.2014.989785"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2004.00225.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01541-15"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080330209"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092300208"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309140200"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2012.01991.x"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.29.1.545"},{"key":"e_1_2_7_49_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1242\/jcs.114.1.3","article-title":"Actin dynamics","volume":"114","author":"Pollard TD","year":"2001","journal-title":"J Cell Sci"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17020215"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod13.5.568"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101071"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2015.07.008"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10203"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.4161\/sgtp.29710"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2015.09.027"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00274-10"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402040112"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.06.083"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.653220"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25044"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080260412"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.18.2295"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2016.03.002"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.1960"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092410111"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.068783"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.22657","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.22657","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.22657","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,16]],"date-time":"2024-06-16T11:41:02Z","timestamp":1718538062000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.22657"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,6]]},"references-count":66,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1002\/mrd.22657"],"URL":"https:\/\/doi.org\/10.1002\/mrd.22657","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,6]]}}}