{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T20:13:03Z","timestamp":1781813583579,"version":"3.54.5"},"reference-count":69,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T00:00:00Z","timestamp":1480291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005187","name":"U.S. Nuclear Regulatory Commission","doi-asserted-by":"publisher","award":["BIOTEK2021"],"award-info":[{"award-number":["BIOTEK2021"]}],"id":[{"id":"10.13039\/100005187","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:label\/><jats:p>Atlantic salmon is a valuable commercial aquaculture species that would benefit economically and environmentally by controlling precocious puberty and preventing escapees from reproducing with wild populations. One solution to both these challenges is the production of sterile individuals by inhibiting the formation of germ cells, but achieving this requires more information on the specific factors that control germ cell formation. Here, we identified and characterized novel factors that are preferentially expressed in Atlantic salmon germ cells by screening for gonad\u2010specific genes using available adult multi\u2010tissue transcriptomes. We excluded genes with expression in tissues other than gonads based on quantity of reads, and then a subset of genes was selected for verification in a multi\u2010tissue PCR screen. Four gonad\u2010specific genes (<jats:italic>bmp15l<\/jats:italic>, <jats:italic>figla<\/jats:italic>, <jats:italic>smc1bl<\/jats:italic>, and <jats:italic>larp6l<\/jats:italic>) were chosen for further characterization, namely: germ cell specificity, investigated by comparing mRNA abundance in wild\u2010type and germ cell\u2010free gonads by quantitative real\u2010time PCR, and cellular location, visualized by in situ hybridization. All four genes were expressed in both testis and ovary, and preferentially within the germ cells of both sexes. These genes may be essential players in salmon germ cell development, and could be important for future studies aiming to understand and control reproduction. <jats:italic>Mol. Reprod. Dev. 84: 76\u201387, 2017. \u00a9 2016 Wiley Periodicals, Inc<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22755","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T11:49:28Z","timestamp":1478519368000},"page":"76-87","source":"Crossref","is-referenced-by-count":17,"title":["<i>bmp15l<\/i>, <i>figla<\/i>, <i>smc1bl<\/i>, and <i>larp6l<\/i> are preferentially expressed in germ cells in Atlantic salmon (<i>Salmo salar<\/i> L.)"],"prefix":"10.1002","volume":"84","author":[{"given":"Lene","family":"Kleppe","sequence":"first","affiliation":[{"name":"Institute of Marine Research Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf Brudvik","family":"Edvardsen","sequence":"additional","affiliation":[{"name":"Institute of Marine Research Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Furmanek","sequence":"additional","affiliation":[{"name":"Institute of Marine Research Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Andersson","sequence":"additional","affiliation":[{"name":"Institute of Marine Research Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e9lie","family":"Juanchich","sequence":"additional","affiliation":[{"name":"Recherches Avicoles Nouzilly France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Wargelius","sequence":"additional","affiliation":[{"name":"Institute of Marine Research Bergen 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