{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T23:11:41Z","timestamp":1771542701063,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,3,24]],"date-time":"2017-03-24T00:00:00Z","timestamp":1490313600000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","award":["1412"],"award-info":[{"award-number":["1412"]}],"id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:label\/><jats:p>Regucalcin is a multi\u2010functional, calcium\u2010binding protein with roles in calcium homeostasis, apoptosis, cell proliferation, and free radical neutralization. Regucalcin is broadly expressed in the male reproductive organs of rat and bovine; here, we report its expression in the reproductive tract of male buffalo\u2014especially in testis, epididymis, seminal vesicle, prostate, and bulbourethral gland of buffalo\u2014as analyzed by real\u2010time PCR, Western blot, and immunolocalization. Regucalcin degradation in seminal plasma, despite its high abundance in vesicular fluid, was demonstrated using recombinant regucalcin co\u2010incubated with buffalo seminal plasma. This depletion of regucalcin appears to be related to its suppressive effect on in vitro sperm capacitation, observed using the chlortetracycline assay after treating buffalo spermatozoa with recombinant protein. Indeed, addition of recombinant regucalcin to capacitating media significantly reduced (<jats:italic>P\u2009<\/jats:italic>&lt;\u20090.05) the percentage of capacitated spermatozoa to 6.1\u2009\u00b1\u20090.6 from 36.4\u2009\u00b1\u20091.8 in the untreated group. Taken together, the wide distribution of regucalcin in male buffaloes, versus its degradation in the seminal plasma and suppressive effects on in vitro capacitation of spermatozoa, indicate its possible anti\u2010capacitation role in the reproductive tract. <jats:italic>Mol. Reprod. Dev.  84: 212\u2013221, 2017. \u00a9 2016 Wiley Periodicals, Inc<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22767","type":"journal-article","created":{"date-parts":[[2016,12,17]],"date-time":"2016-12-17T17:41:40Z","timestamp":1481996500000},"page":"212-221","source":"Crossref","is-referenced-by-count":8,"title":["Regucalcin is widely distributed in the male reproductive tract and exerts a suppressive effect on in vitro sperm capacitation in the water buffalo (<i>Bubalus bubalis<\/i>)"],"prefix":"10.1002","volume":"84","author":[{"given":"Harikrishna","family":"Pillai","sequence":"first","affiliation":[{"name":"Division of Animal Biochemistry Indian Veterinary Research Institute Izatnagar Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashish Mukunda","family":"Shende","sequence":"additional","affiliation":[{"name":"Division of Animal Biochemistry Indian Veterinary Research Institute Izatnagar Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehtab Sing","family":"Parmar","sequence":"additional","affiliation":[{"name":"Division of Physiology and Climatology Indian Veterinary Research Institute Izatnagar Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anjaneya","family":"A","sequence":"additional","affiliation":[{"name":"Division of Pathology Indian Veterinary Research Institute Izatnagar Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sreela","family":"L","sequence":"additional","affiliation":[{"name":"Theriogenology Laboratory Animal Reproduction Gynaecology and Obstetrics National Dairy Research Institute Karnal Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arumugam","family":"Kumaresan","sequence":"additional","affiliation":[{"name":"Theriogenology Laboratory Animal Reproduction Gynaecology and Obstetrics National Dairy Research Institute Karnal Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taru Sharma","family":"G","sequence":"additional","affiliation":[{"name":"Division of Physiology and Climatology Indian Veterinary Research Institute Izatnagar Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjeev Kumar","family":"Bhure","sequence":"additional","affiliation":[{"name":"Division of Animal Biochemistry Indian Veterinary Research Institute Izatnagar Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,3,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod51.6.1238"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016545"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007007626813"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9022297"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2047-2927.2014.00186.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gat030"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3333"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod54.1.138"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5b01337"},{"key":"e_1_2_6_11_1","first-page":"1","article-title":"Regucalcin expression in bovine tissues and its regulation by sex steroid hormones in accessory sex glands","volume":"9","author":"Cucuzza LS","year":"2014","journal-title":"PLoS ONE"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2006.04.028"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)50212-5"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/3.1.1"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080400213"},{"key":"e_1_2_6_16_1","first-page":"427","article-title":"Characterization of protein tyrosine phosphatase activity in rat liver microsomes: Suppressive effect of endogenous regucalcin in transgenic rats","volume":"14","author":"Fukaya Y","year":"2004","journal-title":"Int J Mol Med"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10930-016-9674-x"},{"key":"e_1_2_6_18_1","unstructured":"HarikrishnaP.2016. 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