{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T12:32:18Z","timestamp":1769344338610,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,3,16]],"date-time":"2017-03-16T00:00:00Z","timestamp":1489622400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31301226"],"award-info":[{"award-number":["31301226"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:label\/><jats:p>The epididymis, which connects the testis to vas deferens, plays a crucial role regulating sperm maturation and fertilization. Here, a tamoxifen\u2010inducible <jats:italic>CreER<jats:sup>T2<\/jats:sup><\/jats:italic> recombinase transgenic mouse was generated to study the function of genes in the caput epididymis using the Cre\/LoxP system, which is driven by the 1.8\u2010kb <jats:italic>Lcn5<\/jats:italic> promoter (<jats:italic>Lcn5\u2010CreER<jats:sup>T2<\/jats:sup><\/jats:italic>). Both CRE recombinase and <jats:italic>ER<jats:sup>T2<\/jats:sup><\/jats:italic> mRNA were specifically expressed in the caput epididymis, beginning at postnatal Day 30 and increasing thereafter. Crossing these <jats:italic>Lcn5\u2010CreER<jats:sup>T2<\/jats:sup><\/jats:italic> transgenic mice with <jats:italic>Rosa26; mT\/mG<\/jats:italic> reporter mice, which express membrane\u2010bound GFP (mGFP) only after CRE is active at its genetic locus, resulted in the presence of GFP only in the middle\/distal caput epididymis after tamoxifen induction. Efficiency of the CRE recombinase production in the caput epididymis was dose\u2010 and time\u2010dependent. These tamoxifen\u2010inducible caput epididymis\u2010specific CRE recombinase transgenic mice thus provides a simple approach to modulate epididymal principal cells in vivo, allowing for the genetic investigation of caput epididymis\u2010specific gene functions during sperm maturation. <jats:italic>84: 257\u2013264, 2017. \u00a9 2016 Wiley Periodicals, Inc<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22772","type":"journal-article","created":{"date-parts":[[2016,12,28]],"date-time":"2016-12-28T10:32:50Z","timestamp":1482921170000},"page":"257-264","source":"Crossref","is-referenced-by-count":12,"title":["Expression of tamoxifen\u2010inducible CRE recombinase in <i>Lcn5\u2010CreER<sup>T2<\/sup><\/i> transgenic mouse caput epididymis"],"prefix":"10.1002","volume":"84","author":[{"given":"Juan","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Nanjing Maternity and Child Health Care Hospital Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangxin","family":"Yao","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanfei","family":"Ru","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai P. R. China"},{"name":"Shanghai Institute of Planned Parenthood Research Shanghai P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5124-2673","authenticated-orcid":false,"given":"Shengsong","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture Huazhong Agricultural University Wuhan P. R. China"},{"name":"The Cooperative Innovation Center for Sustainable Pig Production Huazhong Agricultural University Wuhan P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,3,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1381-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601272"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038457"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-16-0268"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmn055"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00256565"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.19.8642-8648.2004"},{"key":"e_1_2_7_9_1","first-page":"39","article-title":"Some aspects of structure and function in the mammalian epididymis","volume":"13","author":"Glover TD","year":"1971","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03282.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.057323"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0768"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.12-205211"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.6.1881"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.12.8282"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20335"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.10.1184"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703445104"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.092403"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.110.085613"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2015.182"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.112.104034"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod50.4.917"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.6.1103"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0478-4"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004106"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.111.013060"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.22772","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.22772","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T17:55:52Z","timestamp":1694886952000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.22772"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1002\/mrd.22772"],"URL":"https:\/\/doi.org\/10.1002\/mrd.22772","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3]]}}}