{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:15:43Z","timestamp":1740104143730,"version":"3.37.3"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,6,19]],"date-time":"2017-06-19T00:00:00Z","timestamp":1497830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31301977"],"award-info":[{"award-number":["31301977"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005196","name":"Chinese Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["2017ywf\u2010zd\u20108"],"award-info":[{"award-number":["2017ywf\u2010zd\u20108"]}],"id":[{"id":"10.13039\/501100005196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:label\/><jats:p>Respiratory distress is a major cause of mortality in cloned neonatal animals, but its pathogenesis remains poorly understood. Here, we used necropsy and histology procedures to evaluate the lungs of cloned neonatal bovines dying of respiratory distress, finding incomplete lung dilation, alveolar collapse, and thickened alveolar walls. Comparison of the transcriptomes between collapsed lungs of cloned bovines and their normal counterparts revealed 1373 differentially expressed genes in collapsed lungs (<jats:italic>p\u2009<\/jats:italic>&lt;\u20090.05, fold change &gt;1.5 or &lt;1.5<jats:sup>\u22121<\/jats:sup>), many of which were associated with surfactant biosynthesis, secretion, transport, recycling, and degradation. ERK\/MAPK and Notch signaling pathways were among the canonical pathways relevant to surfactant homeostasis. Expression of the genes encoding Surfactant protein B (SPB) and Surfactant protein C (SPC)\u2014which control surfactant lipid packing, spreading, and stability\u2014were significantly lower in collapsed lungs of cloned neonates at the transcript (<jats:italic>p\u2009<\/jats:italic>&lt;\u20090.01) and protein levels (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) relative to that in normal lungs. Thus, our results provide an initial view into the changes in gene expression in cloned newborns with lung collapse and respiratory distress, and present a valuable resource for developing novel preventive or therapeutic strategies to reduce the mortality rate of cloned animals and to improve the efficiency of somatic cell nuclear transfer technology.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22836","type":"journal-article","created":{"date-parts":[[2017,5,17]],"date-time":"2017-05-17T13:45:21Z","timestamp":1495028721000},"page":"668-674","source":"Crossref","is-referenced-by-count":2,"title":["Transcriptomic profiling reveals disordered regulation of surfactant homeostasis in neonatal cloned bovines with collapsed lungs and respiratory distress"],"prefix":"10.1002","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8452-7181","authenticated-orcid":false,"given":"Yan","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifan","family":"Rao","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojing","family":"Jiang","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China"},{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linhua","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weihua","family":"Du","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haisheng","family":"Hao","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuling","family":"Jiang","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huabin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuzhu","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,6,19]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_2_1","DOI":"10.1016\/j.theriogenology.2010.11.001"},{"issue":"6","key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1542\/peds.96.6.1046","article-title":"Partial deficiency of surfactant protein B in an infant with chronic lung disease","volume":"96","author":"Ballard P. 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