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Cryoinjuries resulting from freeze\u2010thawing protocols are a major concern with regard to the fertilization capacity of the treated sperm, which is lower than that of liquid\u2010stored semen. Here, we provide an overview of artificial insemination using cryopreserved sperm, and summarize the factors that influence cryopreservation success before, during, and after freeze\u2010thaw (i.e., sperm selection before starting the cryopreservation process, holding time, use of cryoprotectants, and rates of freezing and thawing) and that are driving the identification of biomarkers to predict sensitivity to cryodamage. Three different artificial insemination techniques (conventional or intracervical; intrauterine; and deep intrauterine) are also discussed with regards to their relevance when using frozen\u2010thawed semen. Finally, we review the use of additives to freezing and thawing media, given reports that they may maintain and improve the quality and fertilizing capacity of frozen\u2010thawed sperm. In sum, artificial insemination with frozen\u2010thawed boar sperm can provide reasonable fertility outcomes, if freezable ejaculates, specific additives, and appropriate insemination techniques are used.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22840","type":"journal-article","created":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T12:02:43Z","timestamp":1497355363000},"page":"802-813","source":"Crossref","is-referenced-by-count":122,"title":["Artificial insemination with frozen\u2010thawed boar sperm"],"prefix":"10.1002","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2209-340X","authenticated-orcid":false,"given":"Marc","family":"Yeste","sequence":"first","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Unit of Cell Biology, Department of Biology, Institute of Food and Agricultural Technology University of Girona Girona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan E.","family":"Rodr\u00edguez\u2010Gil","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Unit of Animal Reproduction, Department of Animal Medicine and Surgery, Autonomous University of Barcelona Bellaterra (Cerdanyola del Vall\u00e8s) Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergi","family":"Bonet","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Unit of Cell Biology, Department of Biology, Institute of Food and Agricultural Technology University of Girona Girona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00801.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00744.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.02.026"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2014.07.004"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.06.014"},{"key":"e_1_2_10_7_1","first-page":"553","article-title":"Low dose deep intrauterine insemination of sows with fresh and frozen\u2010thawed spermatozoa","volume":"631","author":"Bathgate R.","year":"2005","journal-title":"Theriogenology"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.01.008"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.06.007"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2012.01999.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.1990.tb00958.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1186\/BF03546945","article-title":"Cryopreservation of boar semen. 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