{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T21:35:44Z","timestamp":1764020144236,"version":"3.37.3"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T00:00:00Z","timestamp":1500336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["HD36239"],"award-info":[{"award-number":["HD36239"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:label\/><jats:p>TAR DNA\u2010binding protein of 43\u2009kDa (TDP\u201043) is an evolutionarily conserved, ubiquitously expressed, multi\u2010functional DNA\/RNA\u2010binding protein with roles in gene transcription, mRNA splicing, stability, transport, micro RNA biogenesis, and suppression of transposons. Aberrant expression of TDP\u201043 in testis and sperm was recently shown to be associated with male infertility, which highlights the need to understand better the expression of TDP\u201043 in the testis. We previously cloned TDP\u201043 from a mouse testis cDNA library, and showed that it functions as a transcriptional repressor and regulates the precise spatiotemporal expression of the <jats:italic>Acrv1<\/jats:italic> gene, which encodes the acrosomal protein SP\u201010, during spermatogenesis. Here, we performed immunoblotting and immunohistochemistry of the mouse testis using four separate antibodies recognizing the amino and carboxyl termini of TDP\u201043. TDP\u201043 is present in the nuclei of germ cells as well as Sertoli cells. TDP\u201043 expression begins in type B\/intermediate spermatogonia, peaks in preleptotene spermatocytes, and becomes undetectable in leptotene and zygotene spermatocytes. Pachytene spermatocytes and early round spermatids again express TDP\u201043, but its abundance diminishes later in spermatids (at steps 5\u20138). Interestingly, two of the four antibodies showed TDP\u201043 expression in spermatids at steps 9\u201310, which coincides with the initial phase of the histone\u2010to\u2010protamine transition. Immunoreactivity patterns observed in the study suggest that TDP\u201043 assumes different conformational states at different stages of spermatogenesis. TDP\u201043 pathology has been extensively studied in the context of neurodegenerative diseases; its role in spermatogenesis warrants further detailed investigation of the involvement of TDP\u201043 in male infertility.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22851","type":"journal-article","created":{"date-parts":[[2017,6,10]],"date-time":"2017-06-10T12:26:38Z","timestamp":1497097598000},"page":"675-685","source":"Crossref","is-referenced-by-count":7,"title":["Immunolocalization of TAR DNA\u2010binding protein of 43\u2009kDa (TDP\u201043) in mouse seminiferous epithelium"],"prefix":"10.1002","volume":"84","author":[{"given":"Hari Prasad","family":"Osuru","sequence":"first","affiliation":[{"name":"Department of Pathology University of Virginia School of Medicine Charlottesville Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patcharin","family":"Pramoonjago","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Virginia School of Medicine Charlottesville Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayuresh M.","family":"Abhyankar","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Virginia School of Medicine Charlottesville Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Swanson","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Virginia School of Medicine Charlottesville Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LaToya Ann","family":"Roker","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Virginia School of Medicine Charlottesville Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Cathro","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Virginia School of Medicine Charlottesville Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4891-8964","authenticated-orcid":false,"given":"Prabhakara P.","family":"Reddi","sequence":"additional","affiliation":[{"name":"Department of Comparative Biosciences University of Illinois at Urbana Champaign Urbana Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705811200"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.04.443"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2660(09)66001-6"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/420978"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.7.1774"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2014.07.009"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2009.06211.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21425"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.03.006"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.166587"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3121"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms10010232"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1134108"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22358"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.6.3584-3596.1995"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)90064-7"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.060438"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-009-9489-9"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD14090"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.03.158"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-15-0239"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2303"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0900688106"}],"container-title":["Molecular 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