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We tested the hypothesis that circulating extracellular vesicle\u2010derived miRNAs might differentiate the pregnancy status of cows that had maintained pregnancy to Day 30 from non\u2010pregnant cows or from those that exhibited embryonic mortality between Days 17 and 30 of gestation. Cows were randomly assigned for artificial insemination with fertile semen (<jats:italic>n<\/jats:italic>\u2009=\u200936) or dead semen (<jats:italic>n<\/jats:italic>\u2009=\u20098; control group) on Day 0 (day of estrus). Blood was collected from all animals on Day 0 and on Days 17 and 24 after artificial insemination. Cows receiving live sperm were retrospectively classified as pregnant on Day 30 (<jats:italic>n<\/jats:italic>\u2009=\u200917) or exhibiting embryonic mortality between Days 17 and 30 (<jats:italic>n<\/jats:italic>\u2009=\u200919). Extracellular vesicles from Day 17 and 24 samples were isolated from serum using ultra\u2010centrifugation, and their presence was confirmed by nanoparticle tracking and Western blot analyses (for CD81) prior to RNA extraction. MicroRNA sequencing was performed on pregnant, embryonic\u2010mortality, and control cows (<jats:italic>n<\/jats:italic>\u2009=\u20094 per day), for a total of 24 independent reactions. In total, 214 miRNAs were identified in serum, 40 of which were novel. Based on differential abundance parameters, we identified 32 differentially abundant loci, representing 27 differentially abundant mature miRNA. At Days 17 and 24, specific miRNAs (e.g., miR\u201025, \u201016b, and \u20103596) were identified that differentiated the pregnancy status. In summary, we identified several circulating extracellular vesicles derived miRNAs that differ in abundance between embryonic mortality and pregnant cows.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22856","type":"journal-article","created":{"date-parts":[[2017,6,23]],"date-time":"2017-06-23T11:36:41Z","timestamp":1498217801000},"page":"731-743","source":"Crossref","is-referenced-by-count":71,"title":["Circulating microRNA as candidates for early embryonic viability in cattle"],"prefix":"10.1002","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2429-0893","authenticated-orcid":false,"given":"Ky G.","family":"Pohler","sequence":"first","affiliation":[{"name":"Department of Animal Science University of Tennessee Knoxville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan A.","family":"Green","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences University of Missouri Columbia 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