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Significant reductions in semen volume, sperm concentration, and total sperm count were observed in diabetic individuals, while transmission electron microscopy revealed that the structure of mitochondria in the tail of sperm from diabetic patients was damaged. Proteins potentially associated with these sperm defects were identified using proteomics. Isobaric tagging for relative and absolute quantitation labeling and high\u2010performance liquid chromatography\u2010tandem mass spectrometry allowed us to identify 357 proteins significantly differentially expressed in diabetic versus control semen (&gt;1.2 or &lt;0.83). According to gene ontology enrichment and pathway analyses, many of these differentially expressed proteins are associated with sperm function, including binding of sperm to the zona pellucida and proteasome function; of particular interest, half of these proteins were related to mitochondrial metabolism. Protein\u2010interaction networks revealed that a decrease in Cystatin C and Dipeptidyl peptidase 4 in the mitochondria may be sources of the decreased motility of sperm from diabetic patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22930","type":"journal-article","created":{"date-parts":[[2017,11,17]],"date-time":"2017-11-17T15:22:38Z","timestamp":1510932158000},"page":"7-16","source":"Crossref","is-referenced-by-count":35,"title":["Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria\u2010related proteins"],"prefix":"10.1002","volume":"85","author":[{"given":"Tian","family":"An","sequence":"first","affiliation":[{"name":"Diabetes Research Center Beijing University of Chinese Medicine Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue\u2010Fen","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Hospital of 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