{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:32:41Z","timestamp":1787495561990,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T00:00:00Z","timestamp":1514332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The National Major Project for Production of Transgenic Breeding","award":["2016ZX08007-002"],"award-info":[{"award-number":["2016ZX08007-002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Intracellular free calcium ([Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ]\n                      <jats:sub>i<\/jats:sub>\n                      ) is essential for oocyte maturation and early embryonic development. Here, we investigated the role of [Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ]\n                      <jats:sub>i<\/jats:sub>\n                      in oocytes from cumulus\u2010oocyte complexes (COCs) with respect to maturation and early embryonic development, using the calcium\u2010buffering agent BAPTA\u2010AM (1,2\u2010bis[2\u2010aminophenoxy]ethane\u2010\n                      <jats:italic>N,N,N\u2032,N\u2032<\/jats:italic>\n                      \u2010tetraacetic acid tetrakis [acetoxymethyl ester]). COCs were graded based on compactness of the cumulus mass and appearance of the cytoplasm, with Grade 1 indicating higher quality and developmental potential than Grade 3. Results showed that: (i) [Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ]\n                      <jats:sub>i<\/jats:sub>\n                      in metaphase\u2010II (MII) oocytes from Grade\u20103 COCs was significantly higher than those from Grade\u20101 COCs, and was significantly reduced by BAPTA\u2010AM; (ii) nuclear maturation of oocytes from Grade\u20103 COCs treated with BAPTA\u2010AM was enhanced compared to untreated COCs; (iii) protein abundance of Cyclin B and oocyte\u2010specific Histone 1 (H1FOO) was improved in MII oocytes from Grade\u20103 COCs treated with BAPTA\u2010AM; (iv) Ca\n                      <jats:sup>2+<\/jats:sup>\n                      transients were triggered in each group upon fertilization, and the amplitude of [Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ]\n                      <jats:sub>i<\/jats:sub>\n                      oscillations increased in the Grade\u20103 group upon treatment with BAPTA\u2010AM, with the magnitude approaching that of the Grade\u20101 group; and (v) cleavage rates and blastocyst\u2010formation rates were improved in the Grade\u20103 group treated with BAPTA\u2010AM compared to untreated controls following in vitro fertilization and parthenogenetic activation. Therefore, BAPTA\u2010AM dramatically improved oocyte maturation, oocyte quality, and embryonic development of oocytes from Grade\u20103 COCs.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mrd.22936","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T05:59:14Z","timestamp":1512453554000},"page":"38-45","source":"Crossref","is-referenced-by-count":6,"title":["BAPTA\u2010AM dramatically improves maturation and development of bovine oocytes from grade\u20103 cumulus\u2010oocyte complexes"],"prefix":"10.1002","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1951-5448","authenticated-orcid":false,"given":"Hongmei","family":"Hu","sequence":"first","affiliation":[{"name":"State Key Laboratory for Agrobiotechnology  College of Biological Sciences China Agricultural University Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianhong","family":"Mo","sequence":"additional","affiliation":[{"name":"College of Life Sciences Chifeng University Chifeng P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Agrobiotechnology  College of Biological Sciences China Agricultural University Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangwei","family":"Fu","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics Breeding and Reproduction Ministry of Agriculture College of Animal Science and Technology China Agricultural University Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6807-8336","authenticated-orcid":false,"given":"Yunpeng","family":"Hou","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Agrobiotechnology  College of Biological Sciences China Agricultural University Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,27]]},"reference":[{"issue":"3","key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1093\/bfgp\/ell020","article-title":"Histone variants\u2010the structure behind the function","volume":"5","author":"Ausio J.","year":"2006","journal-title":"Briefings in Functional Genomics"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2015.10.023"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/27094"},{"issue":"12","key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"3507","DOI":"10.1242\/dev.120.12.3507","article-title":"Spatiotemporal dynamics of intracellular [Ca2+]i oscillations during the growth and meiotic maturation of mouse oocytes","volume":"120","author":"Carroll J.","year":"1994","journal-title":"Development"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1071\/R97080"},{"issue":"20","key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1136\/vr.141.20.522","article-title":"Influence of cumulus cells on in vitro maturation of denuded buffalo oocytes","volume":"141","author":"Das S. 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