{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T07:33:37Z","timestamp":1770881617057,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,23]],"date-time":"2018-03-23T00:00:00Z","timestamp":1521763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["25112009"],"award-info":[{"award-number":["25112009"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["26430100"],"award-info":[{"award-number":["26430100"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:label\/><jats:p>The common marmoset (<jats:italic>Callithrix jacchus<\/jats:italic>) represents a promising nonhuman primate model for the study of human diseases because of its small size, ease of handling, and availability of gene\u2010modified animals. Here, we aimed to devise reproductive technology for marmoset spermatid injection using immature males for a possible rapid generational turnover. Spermatids at each step could be identified easily by their morphology under differential interference microscopy: thus, early round spermatids had a round nucleus with a few nucleolus\u2010like structures and abundant cytoplasm, as in other mammals. The spermatids acquired oocyte\u2010activating capacity at the late round spermatid stage, as confirmed by the resumption of meiosis and Ca<jats:sup>2+<\/jats:sup> oscillations upon injection into mouse oocytes. The spermatids could be cryopreserved efficiently with a simple medium containing glycerol and CELL BANKER\u00ae. Late round or elongated spermatids first appeared at 10\u201312 months of age, 6\u20138 months before sexual maturation. Marmoset oocytes microinjected with frozen\u2013thawed late round or elongated spermatids retrieved from a 12\u2010month\u2010old male marmoset developed to the 8\u2010cell stage without the need for artificial oocyte activation stimulation. Thus, it might be possible to shorten the intergeneration time by spermatid injection, from 2 years (by natural mating) to 13\u201315 months including gestation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrd.22971","type":"journal-article","created":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T16:57:08Z","timestamp":1519232228000},"page":"376-386","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Oocyte\u2010activating capacity of fresh and frozen\u2013thawed spermatids in the common marmoset (<i>Callithrix jacchus<\/i>)"],"prefix":"10.1002","volume":"85","author":[{"given":"Narumi","family":"Ogonuki","sequence":"first","affiliation":[{"name":"RIKEN BioResource Center  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Inoue","sequence":"additional","affiliation":[{"name":"RIKEN BioResource Center  Tsukuba Ibaraki 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