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The significant decrease of apoptotic cumulus cells was detected in patients with chemical and clinical pregnancies as well as live birth among patients\u00a0PCOS and in the tubal infertility group (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). Furthermore, significantly higher implantation rate and also significantly lower cases of early pregnancy loss were observed in the group of oocytes with less apoptotic cumulus cells. Multivariate logistic regression analysis showed that tPNf together with cumulus cell apoptosis were independent prognostic factors of chemical pregnancy, clinical pregnancy rate, and live birth. Time\u2010lapse embryo parameters may not reflect the cumulus cell apoptosis rate. 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