{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T07:09:02Z","timestamp":1787728142922,"version":"build-2784847793"},"reference-count":54,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,11,14]],"date-time":"2018-11-14T00:00:00Z","timestamp":1542153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["BID PICT 2013\u20101495"],"award-info":[{"award-number":["BID PICT 2013\u20101495"]}],"id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007482","name":"Universidad Nacional de Tucum\u00e1n","doi-asserted-by":"publisher","award":["PIUNT 26\/A570"],"award-info":[{"award-number":["PIUNT 26\/A570"]}],"id":[{"id":"10.13039\/501100007482","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008973","name":"University of North Texas","doi-asserted-by":"publisher","award":["PIUNT A616\/1"],"award-info":[{"award-number":["PIUNT A616\/1"]}],"id":[{"id":"10.13039\/100008973","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u03b2\u2010Nerve growth factor (\u03b2\u2010NGF) is a seminal plasma element, responsible for inducing ovulation in camelids. The main organ of \u03b2\u2010NGF production remains nondescript. The aims of this study were to (a) characterize gene expression and protein localization of \u03b2\u2010NGF and its main receptor tyrosine kinase receptor A (TrKA) in the llama male reproductive tract, and (b) determine whether the seminal \u03b2\u2010NGF interacts with ejaculated sperm by localizing \u03b2\u2010NGF and TrKA in epididymal, ejaculated, and acrosome\u2010reacted (AR) sperms and, additionally, by identifying \u03b2\u2010NGF presence in sperm\u2010adsorbed proteins (SAP). Both \u03b2\u2010NGF and TrkA transcripts are widely expressed along the male reproductive tract, with a higher expression level of \u03b2\u2010NGF at prostate (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). \u03b2\u2010NGF immunolabeling was only positive for prostate, whereas TrKA label was present in epithelial and muscular cells of testis, prostate, bulbourethral glands, and epididymis. Using an immunofluorescent technique, \u03b2\u2010NGF was colocalized with TrKA in the middle piece of ejaculated and AR sperm. However, only TrKA was observed in epididymal sperm indicating that \u03b2\u2010NGF could have a seminal origin. This was also confirmed by the identification of four \u03b2\u2010NGF isoforms in SAP. This study extends the knowledge about the participation of \u03b2\u2010NGF\/TrkA in llama reproduction, providing evidence that may have roles in the regulation of sperm physiology.<\/jats:p>","DOI":"10.1002\/mrd.23075","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T04:44:09Z","timestamp":1539751449000},"page":"934-944","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Expression of \u03b2\u2010NGF and high\u2010affinity NGF receptor (TrKA) in llama (<i>Lama glama<\/i>) male reproductive tract and spermatozoa"],"prefix":"10.1002","volume":"85","author":[{"given":"Luciana M.","family":"Sari","sequence":"first","affiliation":[{"name":"Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO), CONICET\u2010UNT, and Instituto de Biolog\u00eda \u201cDr. Francisco D. Barbieri,\u201d Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia, UNT San Miguel de Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"Zampini","sequence":"additional","affiliation":[{"name":"Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO), CONICET\u2010UNT, and Instituto de Biolog\u00eda \u201cDr. Francisco D. Barbieri,\u201d Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia, UNT San Miguel de Tucum\u00e1n Argentina"},{"name":"C\u00e1tedra de Biolog\u00eda Celular y Molecular, Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia, Universidad Nacional de Tucum\u00e1n San Miguel de Tucum\u00e1n Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin E.","family":"Arga\u00f1araz","sequence":"additional","affiliation":[{"name":"Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO), CONICET\u2010UNT, and Instituto de Biolog\u00eda \u201cDr. Francisco D. Barbieri,\u201d Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia, UNT San Miguel de Tucum\u00e1n Argentina"},{"name":"C\u00e1tedra de Biolog\u00eda Celular y Molecular, Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia, Universidad Nacional de Tucum\u00e1n San Miguel de Tucum\u00e1n Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda I.","family":"Carretero","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal, C\u00e1tedra de Teriogenolog\u00eda Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda G.","family":"Fumuso","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal, C\u00e1tedra de Teriogenolog\u00eda Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela E.","family":"Barraza","sequence":"additional","affiliation":[{"name":"Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO), CONICET\u2010UNT, and Instituto de Biolog\u00eda \u201cDr. Francisco D. Barbieri,\u201d Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia, UNT San Miguel de Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Ratto","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Campus Isla Teja Valdivia Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0158-6395","authenticated-orcid":false,"given":"Silvana A.","family":"Apichela","sequence":"additional","affiliation":[{"name":"Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO), CONICET\u2010UNT, and Instituto de Biolog\u00eda \u201cDr. Francisco D. Barbieri,\u201d Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia, UNT San Miguel de Tucum\u00e1n Argentina"},{"name":"C\u00e1tedra de Zootecnia General I, Facultad de Agronom\u00eda y Zootecnia, Universidad Nacional de Tucum\u00e1n El Manantial Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,14]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.04.010"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.4081\/ejh.2014.2363"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20310"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2016-1310"},{"key":"e_1_2_7_1_6_1","unstructured":"Biology of Reproduction. Advance online publication 2018 Source and localization of ovulation\u2010inducing factor\/nerve growth factor in male reproductive tissues among mammalian species"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12319"},{"key":"e_1_2_7_1_8_1","first-page":"383","article-title":"NGF and BDNF long\u2010term variations in the thyroid, testis and adrenal glands of a mouse model of fetal alcohol spectrum disorders","volume":"49","author":"Ceccanti M.","year":"2013","journal-title":"Annali dell\u2019Istituto superiore di sanita"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(97)80071-2"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0045(19990915)41:1<39::AID-PROS6>3.0.CO;2-E"},{"issue":"2","key":"e_1_2_7_1_11_1","first-page":"203","article-title":"Electroejaculation in llama (Lama glama)","volume":"14","author":"Director A.","year":"2007","journal-title":"Journal of Camel Practice and Research"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.05.029"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.23.10984"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00362-9"},{"key":"e_1_2_7_1_15_1","first-page":"293","article-title":"Identification of \u03b2\u2010nerve growth factor in dromedary camel seminal plasma and its role in induction of ovulation in females","volume":"29","author":"Fatnassi M.","year":"2017","journal-title":"Emirates Journal of Food and Agriculture"},{"key":"e_1_2_7_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.02.019"},{"key":"e_1_2_7_1_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.635268"},{"key":"e_1_2_7_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000401)21:6<1145::AID-ELPS1145>3.0.CO;2-Z"},{"key":"e_1_2_7_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.02.016"},{"key":"e_1_2_7_1_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/279160a0"},{"key":"e_1_2_7_1_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1980.tb09663.x"},{"key":"e_1_2_7_1_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb06981.x"},{"key":"e_1_2_7_1_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12837"},{"key":"e_1_2_7_1_24_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.09-219N"},{"key":"e_1_2_7_1_25_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD12039"},{"key":"e_1_2_7_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.05.016"},{"issue":"4","key":"e_1_2_7_1_27_1","first-page":"53","article-title":"Immunocytochemical expression of p75LNGFR and trkA in Leydig cells of the human testis","volume":"41","author":"Koeva Y.","year":"1999","journal-title":"Folia Medica"},{"key":"e_1_2_7_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.06.033"},{"key":"e_1_2_7_1_29_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.22.933"},{"key":"e_1_2_7_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2010.06.002"},{"key":"e_1_2_7_1_31_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-12-0466"},{"key":"e_1_2_7_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490280414"},{"key":"e_1_2_7_1_33_1","first-page":"217","article-title":"Seminal plasma proteins: Functions and interaction with protective agents during semen preservation","volume":"65","author":"Manjunath P.","year":"2007","journal-title":"Society of Reproduction and Fertility supplement"},{"key":"e_1_2_7_1_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy041"},{"key":"e_1_2_7_1_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12609"},{"key":"e_1_2_7_1_36_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD07049"},{"key":"e_1_2_7_1_37_1","doi-asserted-by":"publisher","DOI":"10.14748\/bmr.v10.4"},{"key":"e_1_2_7_1_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150110202"},{"key":"e_1_2_7_1_39_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.117.3.629"},{"key":"e_1_2_7_1_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2154035"},{"key":"e_1_2_7_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00574-X"},{"key":"e_1_2_7_1_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-9-24"},{"issue":"1","key":"e_1_2_7_1_43_1","first-page":"13","article-title":"Increased pulmonary neurotrophin protein expression in idiopathic interstitial pneumonias","volume":"24","author":"Ricci A.","year":"2007","journal-title":"Sarcoidosis, Vasculitis, and Diffuse Lung Diseases"},{"key":"e_1_2_7_1_44_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.4.1123"},{"key":"e_1_2_7_1_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.20767"},{"key":"e_1_2_7_1_46_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3140951"},{"key":"e_1_2_7_1_47_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9961075"},{"key":"e_1_2_7_1_48_1","doi-asserted-by":"publisher","DOI":"10.4236\/jbise.2012.59066"},{"key":"e_1_2_7_1_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00495427"},{"key":"e_1_2_7_1_50_1","doi-asserted-by":"publisher","DOI":"10.4081\/ejh.2009.e29"},{"key":"e_1_2_7_1_51_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.091876"},{"key":"e_1_2_7_1_52_1","first-page":"17","volume-title":"Theriogenology in Camelidae: Anatomy, physiology, pathology and artificial breeding","author":"Tibary A.","year":"1997"},{"key":"e_1_2_7_1_53_1","doi-asserted-by":"publisher","DOI":"10.5603\/FHC.2011.0009"},{"key":"e_1_2_7_1_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02532343"},{"key":"e_1_2_7_1_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22816"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.23075","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.23075","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mrd.23075","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.23075","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T06:41:35Z","timestamp":1694760095000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.23075"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,14]]},"references-count":54,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1002\/mrd.23075"],"URL":"https:\/\/doi.org\/10.1002\/mrd.23075","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,14]]},"assertion":[{"value":"2018-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}