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To investigate the underlying mechanisms for abnormal development of cloned pig fetuses, we compared body weight, amniotic fluid (AF) metabolome, and placental transcriptome between SCNT\u2010 and artificial insemination (AI)\u2010derived pig fetuses. Results showed that the body weight of SCNT pig fetuses was significantly lower than that of AI pig fetuses. The identified differential metabolites between the two groups of AF were mainly involved in bile acids and steroid hormones. The levels of all detected bile acids in SCNT AF were significantly higher than those in AI AF. The increase in the AF bile acid levels in SCNT fetuses was linked with the downregulation of placental bile acid transporter expression and the abnormal development of placental folds (PFs), both of which negatively affected the transfer of bile acids from AF across the placenta into the mother\u2019s circulation. Alteration in the AF steroid hormone levels in cloned fetuses was associated with decreased expression of enzymes responsible for steroid hormone biosynthesis in the placenta. In conclusion, cloned pig fetuses undergo abnormal intrauterine development associated with alteration of bile acid and steroid hormone levels in AF, which may be due to the poor development of PFs and the erroneous expression of bile acid transporters and enzymes responsible for steroid hormone biosynthesis in the placentas.<\/jats:p>","DOI":"10.1002\/mrd.23102","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T03:18:14Z","timestamp":1547003894000},"page":"278-291","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Identification of amniotic fluid metabolomic and placental transcriptomic changes associated with abnormal development of cloned pig fetuses"],"prefix":"10.1002","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7999-8752","authenticated-orcid":false,"given":"Zheng","family":"Ao","sequence":"first","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"},{"name":"Guangdong Provincial Key Laboratory of Agro\u2010animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zicong","family":"Li","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"},{"name":"Guangdong Provincial Key Laboratory of Agro\u2010animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingwang","family":"Wang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"},{"name":"Guangdong Provincial Key Laboratory of Agro\u2010animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengfa","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"},{"name":"Guangdong Provincial Key Laboratory of Agro\u2010animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanmin","family":"Gan","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"},{"name":"Guangdong Provincial Key Laboratory of Agro\u2010animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Wu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"},{"name":"Guangdong Provincial Key Laboratory of Agro\u2010animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Zeng","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, South China Agricultural University Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junsong","family":"Shi","sequence":"additional","affiliation":[{"name":"Wen\u2019s Research Institute, Guangdong Wen\u2019s Foodstuff Group Ltd. 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