{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:15:48Z","timestamp":1740104148822,"version":"3.37.3"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T00:00:00Z","timestamp":1547683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"PAPIIT-UNAM","award":["IN201017","IN200815"],"award-info":[{"award-number":["IN201017","IN200815"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:p>Extracellular purines through specific receptors have been recognized as new regulators of ovarian function. It is known that P2Y2 receptor activity induces theca cell proliferation, we hypothesized that purinergic signaling controls the changes related to hyperthecosis in polycystic ovarian syndrome (PCOS). The aim of this study was to analyze the expression of UTP\u2010sensitive P2Y receptors and their role in theca cells (TC) proliferation in experimentally\u2010induced PCOS (EI\u2010PCOS). In primary cultures of TC from intact rats, all the transcripts of P2Y receptors were detected by polymerase chain reaction; in these cells, UTP (10 \u03bcM) induced extracellular signal\u2010regulated kinases (ERK) phosphorylation. Rats with EI\u2010PCOS showed a reduced expression of P2Y2R in TC whereas P2Y4R did not change. By analyzing ERK phosphorylation, it was determined that P2Y2R is the most relevant receptor in TC. UTP promoted cell proliferation in TC from control but not from EI\u2010PCOS rats. The in silico analysis of <jats:italic>P2yr2<\/jats:italic> promoter indicated the presence of androgen response elements; the stimulation of TC primary cultures with testosterone promoted a significant reduction in the expression of the <jats:italic>P2yr2<\/jats:italic> transcript. We concluded that P2Y2R participates in controlling the proliferative rate of TCs from healthy ovaries, but this regulation is lost during EI\u2010PCOS.<\/jats:p>","DOI":"10.1002\/mrd.23106","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T16:36:37Z","timestamp":1547051797000},"page":"308-318","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Experimental polycystic ovarian syndrome is associated with reduced expression and function of P2Y2 receptors in rat theca cells"],"prefix":"10.1002","volume":"86","author":[{"given":"Ana\u00ed del Roc\u00edo","family":"Campos\u2010Contreras","sequence":"first","affiliation":[{"name":"Departamento de Neurobiolog\u00eda Celular y Molecular Instituto de Neurobiolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico Juriquilla Quer\u00e9taro M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Patricia","family":"Ju\u00e1rez\u2010Mercado","sequence":"additional","affiliation":[{"name":"Departamento de Neurobiolog\u00eda Celular y Molecular Instituto de Neurobiolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico Juriquilla Quer\u00e9taro M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Gonz\u00e1lez\u2010Gallardo","sequence":"additional","affiliation":[{"name":"Unidad de Proteogen\u00f3mica. 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