{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:16:39Z","timestamp":1761581799025,"version":"3.37.3"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T00:00:00Z","timestamp":1549584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["HD42137","HD49430"],"award-info":[{"award-number":["HD42137","HD49430"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006364","name":"Institut National Du Cancer","doi-asserted-by":"publisher","award":["CA034196"],"award-info":[{"award-number":["CA034196"]}],"id":[{"id":"10.13039\/501100006364","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Given attention to both contraception and treatment of infertility, there is a need to identify genes and sequence variants required for mammalian fertility. Recent unbiased mutagenesis strategies have expanded horizons of genetic control of reproduction. Here we show that male mice homozygous for the ethyl\u2010nitroso\u2010urea\u2010induced <jats:italic>ferf1<\/jats:italic> (fertilization failure 1) mutation are infertile, producing apparently normal sperm that does not fertilize oocytes in standard fertilization in vitro fertilization assays. The <jats:italic>ferf1<\/jats:italic> mutation is a single\u2010base change in the <jats:italic>Dnah1<\/jats:italic> gene, encoding an axoneme\u2010associated dynein heavy chain, and previously associated with male infertility in both mice and humans. This missense mutation causes a single\u2010amino\u2010acid change in the DNAH1 protein in <jats:italic>ferf1<\/jats:italic> mutant mice that leads to abnormal sperm clumping, aberrant sperm motility, and the inability of sperm to penetrate the oocyte's zona pellucida; however, the <jats:italic>ferf1<\/jats:italic> mutant sperm is competent to fertilize zona\u2010free oocytes. Taken together, the various mutations affecting the DNAH1 protein in both mouse and human produce a diversity of phenotypes with both subtle and considerable differences. Thus, future identification of the interacting partners of DNAH1 might lead to understanding its unique function among the sperm dyneins.<\/jats:p>","DOI":"10.1002\/mrd.23120","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T09:25:58Z","timestamp":1549617958000},"page":"416-425","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["ENU\u2010induced mutant allele of <i>Dnah1<\/i>, <i>ferf1<\/i>, causes abnormal sperm behavior and fertilization failure in mice"],"prefix":"10.1002","volume":"86","author":[{"given":"Jianjun","family":"Hu","sequence":"first","affiliation":[{"name":"The Jackson Laboratory Bar Harbor Maine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl","family":"Lessard","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory Bar Harbor Maine"},{"name":"Agriculture and Agri\u2010Food Canada Saskatoon Research Development Centre  Saskatchewan Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Longstaff","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory Bar Harbor Maine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilyn","family":"O'Brien","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory Bar Harbor Maine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Palmer","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory Bar Harbor Maine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Reinholdt","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory Bar Harbor Maine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Eppig","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory Bar Harbor Maine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Schimenti","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory Bar Harbor Maine"},{"name":"College of Veterinary Medicine Cornell University Ithaca New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6669-3354","authenticated-orcid":false,"given":"Mary Ann","family":"Handel","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory Bar Harbor Maine"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,8]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_1_2_1","DOI":"10.1371\/journal.pone.0009387"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_3_1","DOI":"10.1016\/j.ajhg.2013.11.017"},{"key":"e_1_2_7_1_4_1","first-page":"1","article-title":"Infertility and impaired fecundity in the United Staes, 1982\u20132010: Data from the National Survey of Family Growth","volume":"67","author":"Chandra A.","year":"2013","journal-title":"National Health Statistics Reports"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_5_1","DOI":"10.1016\/j.cell.2014.02.056"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_6_1","DOI":"10.1093\/humupd\/dmv020"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_7_1","DOI":"10.1146\/annurev-physiol-020911-153339"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_8_1","DOI":"10.1098\/rspb.2014.0296"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_9_1","DOI":"10.1071\/RD12277"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_10_1","DOI":"10.1038\/aja.2010.101"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_11_1","DOI":"10.1002\/(SICI)1098-2795(199904)52:4<445::AID-MRD14>3.0.CO;2-6"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_12_1","DOI":"10.1093\/humrep\/del322"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_13_1","DOI":"10.1159\/000078192"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_14_1","DOI":"10.1093\/humupd\/dmx022"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_15_1","DOI":"10.1002\/cm.21338"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_16_1","DOI":"10.1007\/s11934-018-0802-3"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_17_1","DOI":"10.1093\/hmg\/10.11.1117"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_18_1","DOI":"10.1242\/dev.090613"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_19_1","DOI":"10.1002\/1873-3468.13101"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_20_1","DOI":"10.1093\/biolre\/ioy028"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_21_1","DOI":"10.1093\/biolre\/ioy133"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_22_1","DOI":"10.1258\/la.2009.009074"},{"doi-asserted-by":"publisher","key":"e_1_2_7_1_23_1","DOI":"10.1262\/jrd.11-015"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.23120","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.23120","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mrd.23120","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.23120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T16:43:14Z","timestamp":1694364194000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.23120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,8]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/mrd.23120"],"URL":"https:\/\/doi.org\/10.1002\/mrd.23120","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"type":"print","value":"1040-452X"},{"type":"electronic","value":"1098-2795"}],"subject":[],"published":{"date-parts":[[2019,2,8]]},"assertion":[{"value":"2018-10-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}