{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:48:31Z","timestamp":1790768911474,"version":"4.1.0"},"reference-count":70,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,5,27]],"date-time":"2019-05-27T00:00:00Z","timestamp":1558915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RDCPJ 442137\u201012"],"award-info":[{"award-number":["RDCPJ 442137\u201012"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the context of artificial insemination, male fertility is defined as the ability to produce functional spermatozoa able to withstand cryopreservation. We hypothesized that interindividual variations in fertility depend on the proportion of the fully functional sperm population contained in the insemination dose. The objective of this study was to identify protein markers of the fully functional sperm subpopulation. Insemination doses from four high\u2010fertility (HF) and four low\u2010fertility (LF) bulls with comparable post\u2010thaw quality parameters were selected for proteomic analysis using iTRAQ technology. Thawed semen was centrifuged through a Percoll gradient to segregate the motile (high density [HD]) from the immotile (low density [LD]) sperm populations. Sperm proteins were extracted with sodium deoxycholate and four groups were compared: LD and HD spermatozoa from LF and HF bulls. A total of 498 unique proteins were identified and quantified. Comparison of HD spermatozoa from HF and LF bulls revealed that five proteins were significantly more abundant in the HF group (AK8, TPI1, TSPAN8, OAT, and DBIL5) whereas five proteins were more abundant in the LF group (RGS22, ATP5J, CLU, LOC616319, and CCT5). Comparison of LD spermatozoa from HF and LF bulls revealed that four proteins were significantly more abundant in the HF group (IL4I1, CYLC2, OAT, and ARMC3) whereas 15 proteins were significantly more abundant in the LF group (HADHA, HSP90AA1, DNASE1L3, SLC25A20, GPX5, TCP1, HIP1, CLU, G5E622, LOC616319, HSPA2, NUP155, DPY19L2, SPERT, and SERPINE2). DBIL5, TSPAN8, and TPI1 showed potential as putative markers of the fully functional sperm subpopulation.<\/jats:p>","DOI":"10.1002\/mrd.23174","type":"journal-article","created":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T05:05:17Z","timestamp":1559019917000},"page":"999-1012","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Proteomic markers of low and high fertility bovine spermatozoa separated by Percoll gradient"],"prefix":"10.1002","volume":"86","author":[{"given":"Olivier","family":"D'Amours","sequence":"first","affiliation":[{"name":"D\u00e9partement d'obst\u00e9trique, Gyn\u00e9cologie et Reproduction Centre de Recherche du Centre Hospitalier de l'Universit\u00e9 Laval  Qu\u00e9bec Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9z\u00e9quiel","family":"Calvo","sequence":"additional","affiliation":[{"name":"Proteomic Core Facility Centre de Recherche du Centre Hospitalier de l'Universit\u00e9 Laval, Axe Reproduction, Sant\u00e9 de la m\u00e8re et de l'enfant  Qu\u00e9bec Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Bourassa","sequence":"additional","affiliation":[{"name":"Proteomic Core Facility Centre de Recherche du Centre Hospitalier de l'Universit\u00e9 Laval, Axe Reproduction, Sant\u00e9 de la m\u00e8re et de l'enfant  Qu\u00e9bec Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Vincent","sequence":"additional","affiliation":[{"name":"Department of Research and Development Semex Alliance, L'Alliance Boviteq Inc  Saint\u2010Hyacinthe Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Blondin","sequence":"additional","affiliation":[{"name":"Department of Research and Development Semex Alliance, L'Alliance Boviteq Inc  Saint\u2010Hyacinthe Qu\u00e9bec 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