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We, thus, aimed to quantify the messenger RNA (mRNA) abundance of genes related to cumulus\u2010oocyte complexes (COCs) and embryo quality in Nelore cows (<jats:italic>Bos taurus indicus<\/jats:italic>) submitted to ovarian superstimulation using only FSH (FSH group;<jats:italic>n<\/jats:italic>\u2009=\u200910) or replacement of the last two doses of FSH by eCG (FSH\/eCG group;<jats:italic>n<\/jats:italic>\u2009=\u200910). All animals were slaughtered and the ovarian antral follicles from both groups (10\u201314\u2009mm in diameter) were aspirated for cumulus, oocyte and in vitro embryo production gene expression analysis. The relative mRNA abundance of 96 genes related to COCs development and embryo quality was measured by RT\u2010qPCR. We found that oocytes are more affected by eCG use and that 35 genes involved in lipid metabolism, oxidative stress, transcriptional control, and cellular development were upregulated in the FSH\/eCG group. In blastocysts, lipid metabolism seems to be the main pathway regulated by eCG use. We suggest that these multiple effects could be due to the ability of eCG to bind LHR and FSHR, which could activate multiple signal transduction pathways in the superstimulated ovary, further impacting the transcriptional profile of COCs and blastocysts.<\/jats:p>","DOI":"10.1002\/mrd.23251","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T04:13:57Z","timestamp":1565151237000},"page":"1639-1651","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Equine chorionic gonadotropin drives the transcriptional profile of immature cumulus\u2010oocyte complexes and in vitro\u2010produced blastocysts of superstimulated Nelore cows"],"prefix":"10.1002","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9859-0576","authenticated-orcid":false,"given":"Fernanda Fagali","family":"Franchi","sequence":"first","affiliation":[{"name":"Departamento de Farmacologia Universidade Estadual Paulista (UNESP) Botucatu S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Augusto","family":"Satrapa","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia Universidade Estadual Paulista (UNESP) Botucatu S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4280-9575","authenticated-orcid":false,"given":"Patr\u00edcia Kubo","family":"Fontes","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia Universidade Estadual Paulista (UNESP) Botucatu S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila Helena","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia Universidade Estadual Paulista (UNESP) Botucatu S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Montanari","family":"Razza","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia Universidade Estadual Paulista (UNESP) Botucatu S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabele Picada","family":"Emanuelli","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia Universidade Estadual Paulista (UNESP) Botucatu S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronaldo Luiz","family":"Ereno","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia Universidade Estadual Paulista (UNESP) Botucatu S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson Assun\u00e7\u00e3o","family":"Mareco","sequence":"additional","affiliation":[{"name":"Universidade do Oeste Paulista (UNOESTE) Presidente Prudente S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo F\u00e1bio Gouveia","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas Universidade Estadual Paulista (UNESP) Assis S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciro Moraes","family":"Barros","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia Universidade Estadual Paulista (UNESP) Botucatu S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony C\u00e9sar","family":"de Souza Castilho","sequence":"additional","affiliation":[{"name":"Universidade do Oeste Paulista (UNOESTE) Presidente Prudente S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,6]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(93)90076-4"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0940177"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-10-0248"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/del065"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6101(96)00042-1"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00469.2012"},{"issue":"3","key":"e_1_2_7_1_8_1","first-page":"1260","article-title":"Efeito ben\u00e9fico da substitui\u00e7\u00e3o das duas \u00faltimas doses de pFSH por eCG no protocolo superestimulat\u00f3rio P\u201036 em vacas Nelore","volume":"35","author":"Barcelos A. 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